Regulation of DNA replication in B.subtilis by YabA

YabA 对枯草芽孢杆菌 DNA 复制的调节

• 批准号: 8045423
• 负责人: Houra Merrikh
• 金额: $ 2.99万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-10-03
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8045423
• 关键词: ATP phosphohydrolase; Address; Affect; Bacteria; Bacterial Model; Binding; Biological Assay; Biological Models; Catalytic Domain; Cell division; Cells; Chromatin; Chromosomes; Complex; Cowpox; DNA Microarray Chip; DNA biosynthesis; Escherichia coli; Frequencies; Goals; Gram-Positive Bacteria; Growth; In Vitro; Location; Organism; Pathogenesis; Polymerase; Precipitation; Process; Prokaryotic Cells; Proteins; Regulation; Replication Initiation; Replication Origin; Series; Subgroup; System; Testing; Time; chromatin immunoprecipitation; helicase; human disease; in vitro Assay; in vivo; insight; melting; mutant; pathogenic bacteria; research study; temperature sensitive mutant; transcription factor

DESCRIPTION (provided by applicant): The mechanism(s) used to control the timing and frequency of replication initiation in Gram positive bacteria are unknown. YabA is a negative regulator of replication initiation in B. subtilis, and is conserved in other Gram positive bacteria. The mechanism by which YabA carries out its regulatory function is not yet understood. I found that YabA associates with the chromosomal origin of replication (oriC), and is delivered there at a step following the loading of the replicative helicase. I propose a series of experiments to identify the factor(s) necessary for association of YabA with oriC. I also propose experiments to determine if the subcellular location of YabA and its association with the chromosome changes during the replication cycle. These experiments should provide insights into the timing of YabA's function in inhibition of replication initiation. Finally, I propose several in vivo and in vitro experiments to determine the mechanism of inhibition of replication initiation by YabA.

描述（由申请人提供）：用于控制革兰氏阳性细菌复制启动的时间和频率的机制尚不清楚。 Yaba是枯草芽孢杆菌复制起始的负调节剂，并且在其他革兰氏阳性细菌中保守。 Yaba执行其调节功能的机制尚不清楚。 我发现Yaba与复制的染色体起源（ORIC）相关联，并在复制旋转酶加载后的一步。我提出了一系列实验，以确定Yaba与Oric的关联所需的因素。我还提出了实验，以确定Yaba的亚细胞位置及其与复制周期中染色体变化的关联。这些实验应提供有关Yaba在抑制复制启动方面功能的时机的见解。最后，我提出了几个体内和体外实验，以确定YABA抑制复制起始的机制。