ClC-3 chloride channel regulation of synaptic transmission

ClC-3 氯离子通道对突触传递的调节

• 批准号: 8255962
• 负责人: Laurel Farmer
• 金额: $ 4.18万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8255962
• 关键词: AMPA Receptors; Adult; Age; Animals; Brain; Ca(2+)-Calmodulin Dependent Protein Kinase; Cell membrane; Chemosensitization; Chloride Channels; Chloride Ion; Chlorides; ClC-3 channel; Data; Dependence; Development; Disease; Equilibrium; Event; Excision; Exhibits; Feedback; Glutamates; Goals; HIV; Hippocampus (Brain); Knock-out; Knockout Mice; Learning; Life; Link; Long-Term Potentiation; Maintenance; Mammals; Mediating; Memory; Modeling; Mus; N-Methyl-D-Aspartate Receptors; N-Methylaspartate; Nerve Degeneration; Neurons; Peptides; Perfusion; Phosphorylation; Phosphorylation Site; Positioning Attribute; Postsynaptic Membrane; Preparation; Process; Proteins; Publishing; Regulation; Role; Seizures; Shapes; Slice; Synapses; Synaptic Transmission; Synaptic Vesicles; Synaptic plasticity; Work; calmodulin-dependent protein kinase II; design; inhibitor/antagonist; neuron development; neuronal survival; novel; postnatal; postsynaptic; prevent; receptor; research study; response; synthetic peptide

Long-term potentiation (LTP) and its dependence upon NMDA receptor-mediated Ca[2+] entry has been widely studied as the presumptive mechanism underlying learning and memory. Numerous proteins have been implicated in the induction, maintenance, and modulation of LTP expression. This proposal adds a novel contributor, the Ca[2+]/calmodulin kinase II (CaMKII) gated Cl[-] channel ClC-3, to the regulation of LTP that is unique in its ability to modulate synaptic responses and LTP in an activity- and developmentally-dependent manner. Although ClC-3 is broadly expressed throughout the brain, the ClC-3 knockout mouse shows complete, selective postnatal neurodegeneration of the hippocampus. In fact, multiple lines of ClC-3 knockout mice exhibit similar hippocampal degeneration, suggesting a novel role of this channel in maintaining normal brain function. In support, recent preliminary studies show seizure-like activity in ClC-3 knockout mice; a role for Cl[-] channels, specifically ClC-3, in regulating excitability in the adult brain has not been studied. Prior work has demonstrated that ClC-3 is spatially and functionally linked to the NMDA receptor: NMDA receptordependent Ca[2+] entry, activation of CaMKII, and subsequent phosphorylation/gating of ClC-3 by CaMKII links the two channels via a Ca[2+]-mediated feedback loop. As a result of the shift in the Cl[-] gradient during development, we hypothesize that ClC-3 facilitates excitation and Ca[2+] influx early in development when the equilibrium potential for Cl[-] is depolarizing; conversely, ClC-3 gating suppresses excitation and Ca[2+] influx, thereby restraining the expression of LTP in adulthood when Cl[-] flux is hyperpolarizing. Thus ClC-3 is ideally suited to differentially influence synaptic plasticity as a function of Ca[2+] influx and internal [Cl[-]]. The goal of this application is to characterize the impact of ClC-3 gating on NMDA receptor currents and expression of LTP as a function of the developmentally-regulated Cl[-] gradient. The ClC-3 knockout model allows us to explore the delicate interaction between neuronal development, plasticity, excitation-inhibition balance, and long-term survival ¿ a unifying mechanism likely to be applicable in multiple contexts beyond chloride channels.

长时程增强(LTP)及其对NMDA受体介导的Ca~(2+)内流的依赖已被广泛研究为学习和记忆的可能机制。许多蛋白质参与了LTP表达的诱导、维持和调节。这项建议增加了一个新的贡献者，钙[2+]/钙调蛋白激酶II(CaMKII)门控的CL[-]通道ClC-3，以其独特的能力，以活动和发育依赖的方式调节突触反应和LTP。虽然ClC-3在整个大脑中广泛表达，但ClC-3基因敲除的小鼠在出生后海马区表现出完全的、选择性的神经变性。事实上，CLC-3基因敲除小鼠的多个品系都表现出类似的海马区变性，这表明该通道在维持正常大脑功能方面发挥了新的作用。作为支持，最近的初步研究表明ClC-3基因敲除小鼠的癫痫样活动；氯[-]通道，特别是ClC-3，在调节成人大脑兴奋性方面的作用尚未被研究。先前的工作已经证明ClC-3在空间和功能上与NMDA受体相关：依赖NMDA受体的Ca[2+]进入，激活CaMKII，然后由CaMKII磷酸化/门控ClC-3，通过钙[2+]介导的反馈环连接这两个通道。由于发育过程中Cl[-]梯度的变化，我们假设ClC-3在发育早期，当Cl2[-]的平衡电势是去极化时，促进了激发和Ca[2+]的内流；相反，ClC-3门控抑制了激发和Ca[2+]内流，从而抑制了成年期Cl2[-]通量超极化时LTP的表达。因此，ClC-3非常适合作为Ca[2+]内流和内部[Cl[-]]的函数来不同地影响突触的可塑性。本应用的目的是表征ClC-3门控对NMDA受体电流和LTP表达的影响，作为发育调节的Cl[-]梯度的函数。ClC-3基因敲除模型使我们能够探索神经元发育、可塑性、兴奋-抑制平衡和长期生存之间的微妙相互作用？这一统一的机制可能适用于氯离子通道以外的多种环境。