Characterization of nucleotide-dependent structural rearrangements in atlastin

atlastin 中核苷酸依赖性结构重排的表征

• 批准号: 8252892
• 负责人: Laura Jean Byrnes
• 金额: $ 3.83万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8252892
• 关键词: Accounting; Affect; Affinity; Alleles; Antiviral Agents; Behavior; Binding; Binding Proteins; Biochemical; Biological; Cell physiology; Child; Childhood; Complement; Cytokinesis; DNA Sequence Rearrangement; Defect; Deterioration; Dimerization; Disease; Disease Management; Dynamin; Endocytosis; Endoplasmic Reticulum; Family; GTP-Binding Proteins; GTPase-Activating Proteins; Gene Mutation; Genes; Genetic; Goals; Guanosine Triphosphate; Guanosine Triphosphate Phosphohydrolases; Hereditary Spastic Paraplegia; Heterodimerization; Homodimerization; Homology Modeling; Hot Spot; Human; Hydrolysis; Infant; Inherited; Intervention; Investigation; Kinetics; Lead; Lower Extremity; Membrane; Membrane Fusion; Membrane Protein Traffic; Minority; Missense Mutation; Modeling; Molecular; Molecular Conformation; Motor Neurons; Mutation; Nerve Endings; Nervous system structure; Neurodegenerative Disorders; Neurons; Nucleotides; Nutrient; Organelles; Pathogenesis; Patients; Property; Protein Isoforms; Proteins; Relative (related person); Resolution; Signaling Molecule; Solutions; Spastic Paraplegia, Hereditary, Autosomal Dominant; Structure; Structure-Activity Relationship; Testing; Tubular formation; Variant; Vesicle; axonal degeneration; base; guanylate; infancy; insight; member; mutant; nervous system disorder; novel; outcome forecast; prevent; protein function; protein protein interaction

DESCRIPTION (provided by applicant): Hereditary spastic paraplegia (HSP) is a group of inherited neurological disorders that cause progressive spasticity and weakness in the lower extremities. Mutations in the atlastin-1 gene, one of the major mutational hot spots in the disease, account for ~10% of autosomal dominant HSP cases (subtype SPG3A), and for the majority of cases in infants or children. Defects in this protein results in axonal degeneration in unique sets of neurons, in this case upper motor neurons, yet the molecular mechanism is poorly understood. Atlastin-1 belongs to the dynamin superfamily of large G-proteins, members of which participate in diverse cellular functions, including endocytosis, organelle fusion and fission, cytokinesis, and antiviral activity. On a cellular level, atlastin-1 is involved in generating the tubular endoplasmic reticulum (ER) network by facilitating homotypic membrane fusion. Those dynamin-related G proteins that catalyze homotypic fusion of biological membranes comprise a small minority of this family, and have not been characterized extensively in their structure-function relationship. Structural characterization as well as investigation of partner protein interactions will provide key insights into how and why defects in atlastin-1 result in its pathogenic effects. The first objective will be the structural characterization of atlastin-1 along the nucleotide hydrolysis cycle. This aim will also include the structure determination of HSP-associated mutant variants of the protein in order to reveal pathogenic mechanisms. The second aim focuses on a functional characterization of atlastin-1 mutants using biochemical and biophysical approaches. These studies will complement the crystallographic analyses. By conducting thorough structure-function analyses of wild-type and mutant atlastin-1 variants, a molecular mechanism for disease pathogenesis can be derived, which may provide better means with regard to disease prognosis and intervention.

描述（由申请人提供）：遗传性痉挛性截瘫（HSP）是一组遗传性神经系统疾病，可导致进行性下肢痉挛和无力。atlastin-1基因突变是该疾病的主要突变热点之一，占常染色体显性HSP病例（SPG3A亚型）的约10%，大多数病例发生在婴儿或儿童中。这种蛋白的缺陷会导致特定神经元的轴突变性，在这种情况下是上运动神经元，但其分子机制尚不清楚。Atlastin-1属于大g蛋白的动力蛋白超家族，其成员参与多种细胞功能，包括内吞作用、细胞器融合和裂变、细胞分裂和抗病毒活性。在细胞水平上，atlastin-1通过促进同型膜融合参与了管状内质网（ER）网络的生成。那些催化生物膜同型融合的动力蛋白相关的G蛋白仅占该家族的一小部分，其结构-功能关系尚未被广泛表征。结构表征以及伴侣蛋白相互作用的研究将为atlastin-1缺陷如何以及为什么导致其致病作用提供关键见解。第一个目标将是沿核苷酸水解循环的atlastin-1的结构表征。这一目标还将包括确定热休克蛋白相关突变体的结构，以揭示致病机制。第二个目标侧重于使用生化和生物物理方法的atlastin-1突变体的功能表征。这些研究将补充晶体学分析。通过对野生型和突变型atlastin-1进行深入的结构-功能分析，可以推导出疾病发病的分子机制，为疾病的预后和干预提供更好的手段。