Behavioral and Physiological Phenotyping

行为和生理表型

• 批准号: 8179838
• 负责人: ALEXANDER A BACHMANOV
• 金额: $ 12.77万
• 依托单位: MONELL CHEMICAL SENSES CENTER
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-05 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8179838
• 关键词: Anesthesia procedures; Animal Model; Animals; Anthropology; Applications Grants; Area; Basic Science; Behavior; Behavioral; Biochemistry; Body Weight; Brain; Carbon Dioxide; Chemical Engineering; Chemical Models; Chemicals; Collaborations; Consultations; Data; Data Analyses; Development; Dissection; Ecology; Electrophysiology (science); Endocrinology; Ensure; Equipment; Equipment and Supplies; Experimental Designs; Faculty; Feeding behaviors; Fiber; Flavoring; Funding; Genetic; Genetic Engineering; Genetically Engineered Mouse; Genotype; Goals; Grant; Histology; Human Resources; Immunology; Individual; Inflammation; Institutes; Instruction; Interdisciplinary Study; Laboratories; Maintenance; Maps; Measures; Metabolic; Microbiology; Microscopic; Microscopy; Mission; Molecular Biology; Monitor; Motor Activity; National Institute on Deafness and Other Communication Disorders; Nerve; Neurosciences; Operative Surgical Procedures; Organic Chemistry; Oxygen; Pharmacology; Phenotype; Physiological; Physiology; Production; Protocols documentation; Psychophysics; Public Health; Research; Research Personnel; Resources; Scientist; Services; Smell Perception; System; Taste Perception; Taste Threshold; Techniques; Testing; Tissues; Training; Training Support; Translations; Use Effectiveness; Zoology; base; conditioning; design; drinking behavior; experience; hedonic; improved; instrumentation; irritation; multidisciplinary; nutrition; preference; prototype; repaired; research study; response

SIGNIFICANCE. Many Monell scientists conduct studies with animals and need to characterize their chemosensory phenotypes. They will benefit from the Phenotyping Core in the following ways. 1) This Core will provide a centralized resource of equipment and supplies that will receive dedicated support and regular maintenance. The Core will also centralize labor-intensive tasks, such as making equipment for preference tests. Studies will be designed and/or conducted by Core personnel with specialized expertise in phenotyping techniques. This will be more efficient than replicating these techniques and personnel in individual laboratories. Regular use and service will ensure that equipment is always operational and available for experimentation. 2) The Core will provide access to equipment and facilities (e.g., LabMaster, surgical facility) that are not practical to maintain in individual laboratories. The Core will offer a range of services and experimental designs that are not available in individual laboratories. 3) Scientists with no experience in animal phenotyping will receive training and will have access to equipment and expertise of the Core. This will facilitate their research and help them to collect preliminary data for grant applications. 4) This Research Core will be an integral component of the Core Center. Interactions between the Phenotyping Core and other Research Cores will facilitate collaborative and interdisciplinary studies that are often not feasible for individual laboratories. For example, the Phenotyping and Histology Cores will be used to characterize genetically engineered mice generated from constructs produced using the Molecular Biology Core. In forward genetics studies, the same animals will be examined using the Phenotyping and Genotyping Cores, and the data obtained will be used for chromosomal mapping studies. We estimate that 9 of the 13 R01 grants in our Research Base will use services provided by this Core at moderate to extensive levels. We also expect that 11 current faculty research groups at Monell will use the services provided by this Core. As a result, the Phenotyping Core will improve the efficiency and quality of animal model studies at the Center, accelerate existing NIDCD-funded projects, and advance translation of the results into benefits for public health.

意义许多Monell科学家对动物进行研究，并需要表征它们的化学感受表型。他们将从以下方面受益于表型核心。 1)这一核心将提供一个集中的设备和用品资源，这些设备和用品将得到专门的支持和定期维护。核心还将集中劳动密集型任务，例如制造偏好测试设备。研究将由具有表型分析技术专业知识的核心人员设计和/或进行。这将比在个别实验室复制这些技术和人员更有效。定期使用和维护将确保设备始终运行并可用于实验。 2)核心区将提供设备和设施（例如，LabMaster，外科设施），在单个实验室中维护是不实际的。核心将提供一系列服务和实验设计，这是单个实验室无法提供的。 3)没有动物表型鉴定经验的科学家将接受培训，并将获得核心的设备和专业知识。这将有利于他们的研究，并帮助他们收集初步数据，以申请资助。 4)该研究中心将成为核心中心的一个组成部分。表型核心和其他研究核心之间的相互作用将促进合作和跨学科的研究，这些研究通常对单个实验室来说是不可行的。例如，表型分析和组织学核心将用于表征使用分子生物学核心产生的构建体生成的基因工程小鼠。在正向遗传学研究中，将使用表型和基因分型核心对相同动物进行检查，获得的数据将用于染色体图谱研究。 我们估计，在我们的研究基地的13个R 01赠款中，有9个将使用该核心提供的服务，这些服务将处于中等到广泛的水平。我们还希望Monell现有的11个教师研究小组将使用该核心提供的服务。因此，表型核心将提高该中心动物模型研究的效率和质量，加速现有的NIDCD资助项目，并推动将结果转化为公共卫生效益。