EXPRESSION CORE

表达核心将决定项目I、II和III确定的候选基因表达的发育时间和组织。这些研究将使用免疫组织化学或原位杂交来确定RNA和/或蛋白质的表达模式，以被认为合适的方式为准。 此外，特别感兴趣的基因将使用RCAS-TVA系统进行分析，以获得野生型和显性负性蛋白的组织表达。横隔膜整个支架很难检查，但这个核心的主管和工作人员已经从阿克曼博士那里学到了必要的技术。肺器官培养在实验室已经使用了20年。在过去的8年里，PIS一直在使用RNAi技术。 与果蝇核心一起，RNAi技术将独一无二地用于该计划项目。Loscertales博士常规使用的方案的简要说明将有助于分析CDH候选基因。

The Expression Core will determine the developmental times and tissues in which candidate genes identified by Projects I, II and III are expressed. These studies will determine both RNA and/or protein expression patterns using immunohistochemistry or in situ hybridiation, whichever is deemed appropriate. Further, genes of particular interest will undergo analysis using the RCAS-TVA system for tissue expression of both wildtype and dominant negative proteins. Diaphragm whole mounts are difficult to examine, but the director and staff of this core have learned the necessary techniques from Dr. Akerman. Lung organ culture has been in use in the lab for 20 years. RNAi technology has been used by the Pis for the past 8 years. Together with the Drosophila Core, RNAi technology will be uniquely available for the program project. Brief descriptions of the protocols that Dr. Loscertales uses routinely which will be instrumental in analyzing CDH candidate genes follow.