OKHSC COBRE: DIABETIC ANIMAL CORE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 本项目中的许多研究者在他们的研究中使用糖尿病动物模型。链脲佐菌素（Streptozotocin，STZ）诱导的大鼠或小鼠糖尿病及遗传性糖尿病模型如秋田小鼠、db/db小鼠和Zuker大鼠等是研究糖尿病并发症常用的模型。然而，糖尿病的诱导和监测以及糖尿病动物的长期维持（如发生大多数糖尿病并发症所需的）与大量的常规工作相关，例如注射STZ（其是高毒性化合物）、遗传性糖尿病动物的基因分型、血糖测量、胰岛素注射和定期的临床数据收集。该核心的目标是为糖尿病的诱导提供集中服务， 糖尿病动物的维护和使用以及促进糖尿病研究。过去，我们在合作的基础上建立了一个集中的糖尿病动物使用设施。我们计划扩大现有的糖尿病动物核心设施，并为糖尿病研究社区提供更广泛的服务。核心将为本项目中的所有PJI和导师提供以下服务，无需额外费用：1）。通过STZ注射在大鼠或小鼠或研究者要求的转基因或基因敲除小鼠中诱导糖尿病。2）。对遗传性糖尿病小鼠和大鼠进行繁殖和基因分型。3）。通过测量高血糖监测糖尿病，必要时注射胰岛素。4）。收集并记录糖尿病动物的临床数据，如体重、尿量等。通过测定尿中白蛋白和肌酐浓度监测糖尿病动物的肾功能。6）。根据用户要求提供糖尿病动物专用饲料。7）。在氧诱导的视网膜病变（OIR）模型中诱导视网膜新生血管形成，OIR模型是增殖性糖尿病视网膜病变的常用模型。8）。进行专门的检测以评估糖尿病并发症。9）。组织解剖，建立糖尿病动物组织库，协调糖尿病动物组织共享。这个糖尿病动物核心将协助PJIs在他们的项目，并减少他们的日常工作，诱导和维护糖尿病动物。糖尿病动物组织的协调共享也将大大减少动物模型的总体预算。除了目前的PJIs和未来的PJIs，这个核心也将有利于导师和整个糖尿病研究社区在OUHSC。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Many investigators in this project use diabetic animal models in their research. Streptozotocin (STZ)-induced diabetes in rats or mice and genetic diabetes models such as Akita mice, db/db mice and Zuker rats, etc, are commonly used models for studying diabetic complications. However, induction and monitoring of diabetes and maintenance of diabetic animals for long durations, as required for most diabetic complications to occur, are associated with tremendous amounts of routine work, such as injection of STZ which is a highly toxic compound, genotyping of genetic diabetic animals, blood glucose measurement, insulin injection and regular clinical data collection. The goal of this Core is to provide a centralized service for induction of diabetes, maintenance and use of diabetic animals and to facilitate diabetic research. In the past, we have formed a centralized diabetic animal use facility on a collaboration basis. We plan to expand this existing diabetic animal core facility and provide broader services to diabetic research community. The Core will provide the following services to all PJIs and Mentors in this project with no additional fees: 1). To induce diabetes by STZ injection in rats or mice or in transgenic or gene knockout mice required by investigators. 2). To breed and genotype genetic diabetic mice and rats. 3). To monitor diabetes by measuring hyperglycemia and inject insulin when necessary. 4). To collect and record clinical data from diabetic animals, such as body weight, urine volume, etc. 5). To monitor renal functions of diabetic animals by measuring albumin and creatinine concentrations in the urine. 6). To provide special diet for diabetic animals upon request by users. 7). To induce retinal neovascularization in the oxygen-induced retinopathy (OIR) model, a commonly used model for proliferative diabetic retinopathy. 8). To perform specialized assays to evaluate diabetic complications. 9). To dissect tissues, establish a tissue bank of diabetic animals and coordinate sharing of tissues from diabetic animals. This Diabetic Animal Core will assist the PJIs in their projects and reduce their routine work in induction and maintenance of diabetic animals. The coordinated sharing of diabetic animal tissues will also substantially reduce the overall budget for animal models. In addition to the current PJIs and future PJIs, this Core will also benefit the mentors and entire diabetes research community at OUHSC.