OKHSC COBRE: DIABETIC ANIMAL CORE

OKHSC COBRE：糖尿病动物核心

• 批准号: 8167966
• 负责人: Jian-Xing Ma
• 金额: $ 33.11万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167966
• 关键词: Albumins; Animal Model; Animals; Biological Assay; Blood Glucose; Body Weight; Breeding; Budgets; Clinical Data; Collaborations; Communities; Complications of Diabetes Mellitus; Computer Retrieval of Information on Scientific Projects Database; Core Facility; Creatinine; Data Collection; Diabetes Mellitus; Diabetic Diet; Diabetic mouse; Fees; Funding; Future; Genes; Genetic; Genotype; Goals; Grant; Hyperglycemia; Injection of therapeutic agent; Institution; Insulin; Knockout Mice; Maintenance; Measurement; Measures; Mentors; Modeling; Monitor; Mus; Oxygen; Poison; Rattus; Renal function; Research; Research Personnel; Resources; Retinal Diseases; Retinal Neovascularization; Services; Source; Streptozocin; Streptozocin Diabetes; Study models; Tissue Banking; Tissue Banks; Tissues; Transgenic Organisms; United States National Institutes of Health; Urine; Work; animal tissue; base; db/db mouse; diabetes mellitus genetics; diabetic; proliferative diabetic retinopathy

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Many investigators in this project use diabetic animal models in their research. Streptozotocin (STZ)-induced diabetes in rats or mice and genetic diabetes models such as Akita mice, db/db mice and Zuker rats, etc, are commonly used models for studying diabetic complications. However, induction and monitoring of diabetes and maintenance of diabetic animals for long durations, as required for most diabetic complications to occur, are associated with tremendous amounts of routine work, such as injection of STZ which is a highly toxic compound, genotyping of genetic diabetic animals, blood glucose measurement, insulin injection and regular clinical data collection. The goal of this Core is to provide a centralized service for induction of diabetes, maintenance and use of diabetic animals and to facilitate diabetic research. In the past, we have formed a centralized diabetic animal use facility on a collaboration basis. We plan to expand this existing diabetic animal core facility and provide broader services to diabetic research community. The Core will provide the following services to all PJIs and Mentors in this project with no additional fees: 1). To induce diabetes by STZ injection in rats or mice or in transgenic or gene knockout mice required by investigators. 2). To breed and genotype genetic diabetic mice and rats. 3). To monitor diabetes by measuring hyperglycemia and inject insulin when necessary. 4). To collect and record clinical data from diabetic animals, such as body weight, urine volume, etc. 5). To monitor renal functions of diabetic animals by measuring albumin and creatinine concentrations in the urine. 6). To provide special diet for diabetic animals upon request by users. 7). To induce retinal neovascularization in the oxygen-induced retinopathy (OIR) model, a commonly used model for proliferative diabetic retinopathy. 8). To perform specialized assays to evaluate diabetic complications. 9). To dissect tissues, establish a tissue bank of diabetic animals and coordinate sharing of tissues from diabetic animals. This Diabetic Animal Core will assist the PJIs in their projects and reduce their routine work in induction and maintenance of diabetic animals. The coordinated sharing of diabetic animal tissues will also substantially reduce the overall budget for animal models. In addition to the current PJIs and future PJIs, this Core will also benefit the mentors and entire diabetes research community at OUHSC.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 该项目中的许多研究人员在研究中使用糖尿病动物模型。大鼠或小鼠的链蛋白酶（STZ）诱导的糖尿病以及遗传糖尿病模型，例如Akita小鼠，DB/DB小鼠和Zuker大鼠等，是研究糖尿病并发症的常用模型。但是，由于大多数糖尿病并发症的发生所需，糖尿病的诱导和监测与糖尿病动物的长期持续时间有关，与大量的常规工作有关，例如注射STZ，这是剧毒的化合物，是遗传性糖尿病动物的基因分型，遗传性糖尿病的基因分型，血糖糖测量，胰岛素临床培训，常规临床数据收集。该核心的目的是为诱导糖尿病提供集中式服务， 维持和使用糖尿病动物并促进糖尿病研究。过去，我们在协作的基础上建立了集中式糖尿病动物使用设施。我们计划扩大这种现有的糖尿病动物核心设施，并为糖尿病研究社区提供更广泛的服务。核心将为该项目的所有PJI和导师提供以下服务，而没有额外的费用：1）。在大鼠，小鼠或研究人员所需的转基因或基因敲除小鼠中，通过STZ注射诱导糖尿病。 2）。繁殖和基因型遗传糖尿病小鼠和大鼠。 3）。在必要时测量高血糖和注射胰岛素，以监测糖尿病。 4）。从糖尿病动物中收集和记录临床数据，例如体重，尿量等。5）。通过测量尿液中的白蛋白和肌酐浓度来监测糖尿病动物的肾功能。 6）。根据用户要求提供特殊的糖尿病动物饮食。 7）。在氧诱导的视网膜病变（OIR）模型中诱导视网膜新血管形成，这是一种常用的增殖性糖尿病性视网膜病模型。 8）。进行专门测定以评估糖尿病并发症。 9）。要剖析组织，请建立一个糖尿病动物的组织库，并从糖尿病动物坐标组织。这种糖尿病动物核心将有助于PJIS进行项目，并减少其在糖尿病动物诱导和维持方面的常规工作。糖尿病动物组织的协调共享还将大大减少动物模型的总体预算。除了当前的PJIS和未来的PJI外，该核心还将使OUHSC的导师和整个糖尿病研究社区受益。