ROLE OF COLONIC EPITHELIAL CELLS IN CITROBACTER INFECTION IN MICE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 腹泻每年影响全球约40亿人，估计死亡人数约为200万。大肠杆菌（E.大肠杆菌），附着在肠道内壁的表面，并导致其破坏，是全球感染性腹泻的常见原因，特别是在2岁以下的儿童中。自E.啮齿柠檬酸杆菌是一种在小鼠中引起自然疾病的细菌，其疾病的临床进展与人类大肠杆菌非常相似。大肠杆菌感染;用作体内模型。与人类一样，小鼠对这些细菌的易感性取决于年龄组（年轻人比成年人受影响更严重）和遗传背景。在小鼠中的许多研究表明，免疫状态对疾病的严重程度或病程的影响很小。我们的研究旨在了解肠上皮细胞衬里在确定疾病严重程度和病程中的作用。上皮细胞是理想的靶点，因为它们不仅在肠道中提供物理屏障，而且还积极参与控制对感染因子的局部抗性。我们将评估感染C.结果将有助于我们了解上皮细胞在决定人类和动物细菌性口腔疾病的易感性和严重性方面的作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Diarrhea affects approximately 4 billion people per year worldwide with number of deaths estimated to be around 2 million. Escherichia coli (E. coli) that attach to the surface of the intestinal lining and cause its destruction, are common causes for infectious diarrhea worldwide, especially in children less than 2 years of age. Since E. coli does not cause clinical illness in mice, Citrobacter rodentium a bacteria that causes natural disease in mice with clinical progression of illness that closely resembles human E. coli infections; is used as an in-vivo model. As in humans, the susceptibility of mice to these bacteria is dependent of age group (the young more severely affected than the adults), and genetic background. Many studies in mice have shown that the immune status has minimal effect on the severity or course of the disease. Our study is aimed at understanding the role of epithelial cell lining of the intestines in defining the severity and course of disease. Epithelial cells are ideal target, since they not only provide a physical barrier in the intestine, but also actively part-take in controlling the local resistance to infectious agents. We will evaluate structural and functional changes in epithelial cells following infection with C. rodentium, and the results will help us understand the role of epithelial cells in determining the susceptibility and severity of bacterial diarrheal illnesses of humans and animals.