STUDY OF THE UNDERLYING FACTORS THAT SHAPE ENZYME PROPERTIES IN ORGANIC SOLVENT

有机溶剂中影响酶性质的基本因素的研究

基本信息

  • 批准号:
    8167849
  • 负责人:
  • 金额:
    $ 23.53万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2010
  • 资助国家:
    美国
  • 起止时间:
    2010-06-01 至 2011-05-31
  • 项目状态:
    已结题

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The usefulness of enzymatic catalysis in organic solvents in introducing chirality to key biologically relevant compounds is welt recognized. However, there are still major drawbacks in such applications which preclude the use of these biocatalysts to their full potential. Particular liabilities are the low enzyme activity observed under nonaqueous conditions (as compared to their natural aqueous medium), and the lack of predictability of the enzymes' selectivity and enantioselectivity. As a consequence, a trial and error approach remains the most effective method to achieve the desired product outcome. The reduced enzyme activity in non-aqueous media has been linked to several factors (substrate's desolvation, enzyme flexibility, and pH dependence) as well as structural perturbations, the ionization-state of the catalytic triad residues, and possible aggregation of an enzyme in organic solvents. All of these parameters depend on the organic solvent used as the medium, and to a lesser extent to the mode of enzyme preparation. Similarly, an enzyme's selectivity and enantioselectivity are also solvent dependent and have been mainly attributed to its flexibility and its structural integrity (organic solvents shape both the enzyme flexibility and its structure). Our contributions to this field during the last 4 years have included (a) a new method to activate enzymes; is (b) evidence of the relationship between the structural integrity and enantioselectivity of subtilisin; (c) identified solvents which are detrimental to an enzyme's structure;is (d) we showed a relationship between flexibility and activity, (e) showed the effect of crown ethers on structure and activity, and (f) we also demonstrated that subtilisin Carlsberg is not stable in organic solvents as first thought. The goal of this proposal is to determine, analyze, and understand the crucial parameters that decide the outcome of any reaction catalyzed by an enzyme in organic solvents. The simple question, for example, as to why subtilisin Carlsberg is more active and enantioselective in tetrahydrofuran than in acetonitrile cannot be readily answered with the current state of knowledge. This knowledge gap will be filled by the proposed research especially due to its scope and multidisciplinary character combining experimental and theoretical methods. The following areas wilt be studied in detail at the experimental and theoretical level: (a) the structural integrity of an enzyme in organic solvents, (b) changes in a suspended enzyme powder's morphology as it might relate to its activity and stability in non-aqueous media, and (c) the mechanism of proton swapping and the role of the active site imidazole (in serine proteases) of reactions catalyzed in neat organic solvents. The realization of the following specific aims will satisfy the principal goal of this research. - To study the different factors that influence enzyme enantioselectivity and to determine for each factor its relative contribution. To accomplish this, a set of theoretical calculations and experiments will be conducted on enzyme-substrate systems spanning the factor-enantioselectivity property space. - To study how the morphology of an enzyme powder is affected by the organic solvents in which it is suspended, and how this relates to the enzyme's activity and stability in this media. The morphology of the suspended enzyme will be characterized using fractal analysis and scanning electron microscopy (SEM). - To determine if the low enzyme activity in different organic solvents is related to the acidity/basicity of the active-site histidine. This will involve: (a) the use of NMR spectroscopy, (b) the modeling of the proton shuffling in the active site to obtain the potential energy curves and to relate that to the possible pKa changes that might occur in different solvents, and (c) to study the catalytic role of the active site histidine in organic solvents using a series of inhibitors. - To study the mechanism of enzyme inactivation in organic solvents by kinetic and mass spectrometry, fluorescence, circular dichroism and diffuse reflectance infra-red. - To study new methods to activate and stabilize enzymes in organic solvents.
这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者(PI)可能从另一个NIH来源获得了主要资金, 因此可以在其他CRISP条目中表示。所列机构为 研究中心,而研究中心不一定是研究者所在的机构。 有机溶剂中的酶催化在将手性引入关键生物相关化合物中的有用性是众所周知的。然而,在这些应用中仍然存在主要的缺点,这妨碍了这些生物催化剂充分发挥其潜力。特别不利的是在非水条件下观察到的低酶活性(与它们的天然水性介质相比),以及缺乏酶的选择性和对映体选择性的可预测性。因此,试错法仍然是实现预期产品结果的最有效方法。非水介质中酶活性的降低与几个因素(底物的去溶剂化、酶的灵活性和pH依赖性)以及结构扰动、催化三联体残基的电离状态和酶在有机溶剂中可能的聚集有关。所有这些参数取决于用作介质的有机溶剂,并且在较小程度上取决于酶制备的模式。同样,酶的选择性和对映选择性也取决于溶剂,并且主要归因于其灵活性和结构完整性(有机溶剂塑造酶的灵活性和结构)。在过去的四年中,我们在这一领域的贡献包括:(a)一种新的激活酶的方法;(B)枯草杆菌蛋白酶的结构完整性和对映体选择性之间关系的证据;(c)鉴定对酶结构有害的溶剂;(d)我们显示了柔性和活性之间的关系,(e)显示了冠醚对结构和活性的影响,和(f)我们还证明了枯草杆菌蛋白酶Carlsberg在有机溶剂中不稳定。该提案的目标是确定,分析和理解决定有机溶剂中酶催化的任何反应结果的关键参数。例如,为什么嘉士伯枯草杆菌蛋白酶在四氢呋喃中比在乙腈中更有活性和对映选择性,这个简单的问题就不能用目前的知识水平来回答。这一知识空白将填补拟议的研究,特别是由于其范围和多学科性质相结合的实验和理论方法。将在实验和理论层面上详细研究以下领域:(a)酶在有机溶剂中的结构完整性,(B)悬浮酶粉末形态的变化,因为它可能与其在非水介质中的活性和稳定性有关,以及(c)质子交换的机理和活性中心咪唑的作用(在丝氨酸蛋白酶中)在纯有机溶剂中催化的反应。实现以下具体目标将满足本研究的主要目标。 - 研究影响酶对映体选择性的各种因素,并确定各因素的相对贡献。为了实现这一点,一组理论计算和实验将进行酶底物系统跨越因子对映体选择性属性空间。 - 研究酶粉末的形态如何受到其悬浮的有机溶剂的影响,以及这与酶在该介质中的活性和稳定性的关系。悬浮酶的形态将使用分形分析和扫描电子显微镜(SEM)进行表征。 - 确定酶在不同有机溶剂中的低活性是否与活性位点组氨酸的酸碱性有关。这将涉及:(a)核磁共振的使用 (B)模拟活性中心的质子重排,得到势能曲线,并将其与不同溶剂中可能发生的pKa变化联系起来,(c)使用一系列抑制剂研究活性中心组氨酸在有机溶剂中的催化作用。 - 采用动力学、质谱、荧光、圆二色谱和漫反射红外光谱等方法研究有机溶剂中酶的失活机理。 - 研究在有机溶剂中激活和稳定酶的新方法。

项目成果

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GABRIEL Luis BARLETTA其他文献

GABRIEL Luis BARLETTA的其他文献

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{{ truncateString('GABRIEL Luis BARLETTA', 18)}}的其他基金

Novel Gold nanocarriers conjugates for microRNA delivery in ovarian cancer
新型金纳米载体缀合物用于卵巢癌中的 microRNA 递送
  • 批准号:
    9977255
  • 财政年份:
    2018
  • 资助金额:
    $ 23.53万
  • 项目类别:
Novel Gold nanocarriers conjugates for microRNA delivery in ovarian cancer
新型金纳米载体缀合物用于卵巢癌中的 microRNA 递送
  • 批准号:
    10225464
  • 财政年份:
    2018
  • 资助金额:
    $ 23.53万
  • 项目类别:
HYDROLASE STABILITY ENHANCEMENT AND ITS APPLICATION TO SIRNA
水解酶稳定性增强及其在 SIRNA 中的应用
  • 批准号:
    8360149
  • 财政年份:
    2011
  • 资助金额:
    $ 23.53万
  • 项目类别:
STUDY OF THE UNDERLYING FACTORS THAT SHAPE ENZYME PROPERTIES IN ORGANIC SOLVENT
有机溶剂中影响酶性质的基本因素的研究
  • 批准号:
    7960048
  • 财政年份:
    2009
  • 资助金额:
    $ 23.53万
  • 项目类别:
STUDY OF THE UNDERLYING FACTORS THAT SHAPE ENZYME PROPERTIES IN ORGANIC SOLVENT
有机溶剂中影响酶性质的基本因素的研究
  • 批准号:
    7720862
  • 财政年份:
    2008
  • 资助金额:
    $ 23.53万
  • 项目类别:
Dynamics and Function Relationships of Hydrolases in Organic Solvents
有机溶剂中水解酶的动力学和功能关系
  • 批准号:
    7288966
  • 财政年份:
    2007
  • 资助金额:
    $ 23.53万
  • 项目类别:
STUDY OF THE UNDERLYING FACTORS THAT SHAPE ENZYME PROPERTIES IN ORGANIC SOLVENT
有机溶剂中影响酶性质的基本因素的研究
  • 批准号:
    7610156
  • 财政年份:
    2007
  • 资助金额:
    $ 23.53万
  • 项目类别:
STUDY OF THE UNDERLYING FACTORS THAT SHAPE ENZYME PROPERTIES IN ORGANIC SOLVENT
有机溶剂中影响酶性质的基本因素的研究
  • 批准号:
    7381560
  • 财政年份:
    2006
  • 资助金额:
    $ 23.53万
  • 项目类别:
STUDY OF THE UNDERLYING FACTORS THAT SHAPE ENZYME PROPERTIES IN ORGANIC SOLVENTS
研究有机溶剂中影响酶性质的基本因素
  • 批准号:
    7170784
  • 财政年份:
    2005
  • 资助金额:
    $ 23.53万
  • 项目类别:
ACTIVITY, STABILITY, MECHANISM OF HYDROLASES
水解酶的活性、稳定性和机制
  • 批准号:
    6972463
  • 财政年份:
    2004
  • 资助金额:
    $ 23.53万
  • 项目类别:

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