CELLULAR AND MOLECULAR CHANGES ASSOCIATED WITH REPRODUCTIVE HEALTH

与生殖健康相关的细胞和分子变化

• 批准号: 8167857
• 负责人: Jennifer Luz Barreto Estrada
• 金额: $ 16.05万
• 依托单位: UNIVERSITY OF PUERTO RICO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167857
• 关键词: Address; Adolescent; Affective; Agonist; Amygdaloid structure; Anabolic steroids; Androgen Analogues; Androgens; Anxiety; Behavior; Behavioral; Biochemical; Brain; Brain region; Cells; Complex; Computer Retrieval of Information on Scientific Projects Database; Cultured Cells; Data; Endorphins; Event; Funding; Gonadal Steroid Hormones; Grant; Growth Factor; Growth Factor Receptors; Hormonal; In Vitro; Institution; Knowledge; Medial; Mediating; Mental Depression; Mental disorders; Methyltestosterone; Molecular; Neuropeptides; Neurosecretory Systems; Opioid; Peptides; Physiological; Preoptic Areas; Puberty; Radioimmunoassay; Rattus; Regulation; Reproduction; Reproductive Behavior; Reproductive Health; Research; Research Personnel; Resources; Role; Source; Symptoms; System; Testosterone; United States National Institutes of Health; base; critical developmental period; immunocytochemistry; in vivo; mRNA Expression; receptor; reproductive; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Sex steroids in the brain regulate reproductive behaviors and have regulatory functions beyond the influence on reproduction. For instance, natural and synthetic androgens can induce psychiatric disorders such as anxiety and depression. Knowledge that androgens can modulate specific cellular substrates such as neuropeptides and growth factors has raised relevant questions regarding the cellular mechanisms responsible for the observed behavioral changes under physiological or abused concentrations. Neuropeptides have been associated with reproductive and anxiety-related behaviors. NPY and opioids, are highly expressed in brain regions that mediate reproductive behaviors and anxiety, but also they have been shown to be under hormonal control. However, the cellular events implied in these behaviors are poorly understood and have been addressed mainly in vivo. This proposal studies the biochemical basis underlying reproductive and anxietyrelated behaviors using an in vitro system. Isolated cells from the medial preoptic area and amygdala complex, will be exposed to testosterone and the anabolic androgenic steroid (AAS), 17¿-methyltestosterone. Cultured cells will be isolated from peripubertal rats, since puberty is a critical developmental period susceptible to hormonal and behavioral changes, and for the alarming increase in adolescents misusing AAS. Immunocytochemistry and radioimmunoassay for NPY and _-endorphin will be used to quantify IR-cells and peptide levels, respectively. Also, mRNA expression for growth factor receptors will be determined. The presence of growth factors and their receptors in the brain might suggest an important role in peptide regulation and therefore, in neuroendocrine function and anxiety. Finally, neuropeptide agonist and antagonists will be centrally infused in vivo to brain regions associated with reproductive and affective behaviors. This aim will encompass a proof of concept between the in vitro and in vivo experiments. These studies will provide critical data regarding the biochemical aspects of psychiatric symptoms associated with androgen exposure.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 大脑中的性类固醇调节生殖行为，并具有调节功能 超越了对生殖的影响。例如，天然和合成的雄激素可以 引发焦虑和抑郁等精神障碍。了解雄激素可以 调节特定的细胞底物，如神经肽和生长因子 关于导致观察到的行为的细胞机制的相关问题 在生理或滥用浓度下的变化。神经肽被认为与 有生殖和焦虑相关行为。NPY和阿片类药物在 调节生殖行为和焦虑的大脑区域，但它们也一直是 显示出在荷尔蒙控制下。然而，这些行为中隐含的细胞事件 人们对此知之甚少，而且主要是在体内解决的。这项建议研究了 体外研究生殖和焦虑相关行为的生化基础 系统。从内侧视前区和杏仁复合体分离的细胞将暴露于 睾酮和合成代谢类雄激素(AAS)，17-甲基睾酮。文化修养 将从青春期大鼠身上分离细胞，因为青春期是一个关键的发育期 容易受到荷尔蒙和行为变化的影响，以及青少年数量惊人的增加 滥用原子吸收光谱。神经肽Y和β-内啡肽的免疫细胞化学和放射免疫分析 分别用于IR细胞和多肽水平的定量。此外，生长发育相关基因的表达 因子受体将被确定。血管内皮细胞生长因子及其受体的存在 大脑可能在肽调节中发挥重要作用，因此在神经内分泌中也可能发挥重要作用 功能和焦虑。最后，神经肽激动剂和拮抗剂将集中在 活体到大脑与生殖和情感行为相关的区域。这一目标将 包括体外和体内实验之间的概念证明。这些研究将 提供有关相关精神症状的生化方面的重要数据 与雄激素接触有关。