COBRE: LSU: CELL BIOLOGY AND TISSUE CULTURE CORE RESOURCE MODULE

COBRE：LSU：细胞生物学和组织培养核心资源模块

• 批准号: 8167390
• 负责人: JIAN-GUO CUI
• 金额: $ 4.38万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-01 至 2011-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167390
• 关键词: Antibiotics; Arts; Cell Culture Techniques; Cell Line; Cells; Cellular biology; Centers of Research Excellence; Computer Retrieval of Information on Scientific Projects Database; Culture Media; Ensure; Faculty; Funding; Grant; Housing; Individual; Institution; Liquid substance; Mammalian Cell; Nitrogen; Participant; Postdoctoral Fellow; Powder dose form; Research; Research Personnel; Resources; Solutions; Source; Sterility; Techniques; United States National Institutes of Health; Work; established cell line; graduate student; salt balance; tissue culture

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This core module has four primary functions. It maintains established cell lines and provides investigators with the cells needed for their experimental work. It operates a cell culture media kitchen; most of the common cell culture media plus Dulbecco's balanced salt solution are prepared from commercially available powder mixtures, filter sterilized, sterility checked and stored for individual use. it houses and maintains a bank of liquid nitrogen dewars for the storage of cell lines. Finally, it assists COBRE participants, graduate students, post-doctoral felows, research associates and faculty in the art of mammalian cell culture with emphsis on sterile technique to ensure the ability of performing antibiotic-free cell culture.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 这个核心模块有四个主要功能。它维持已建立的细胞系，并为研究人员提供实验工作所需的细胞。它经营一个细胞培养基厨房;大多数常见的细胞培养基加上Dulbecco的平衡盐溶液是从市售的粉末混合物制备的，过滤灭菌，无菌检查和储存供个人使用。它容纳和维持一个液氮杜瓦瓶库，用于储存细胞系。最后，它协助COBRE参与者，研究生，博士后研究员，研究人员和教师在哺乳动物细胞培养的艺术，强调无菌技术，以确保执行无菌细胞培养的能力。