Project 5: Phytoremediation to Degrade Airborne PCB Congeners from Soil

项目5.项目5的总体目标是提供工程研究（非生物医学），以修复含有可能暴露于人类的空气中的多氯联苯同系物的场地。具体而言，它是为了确定植物是否可以提供原位植物修复多氯联苯同系物从空气和其他空气传播的来源，如疏浚沉积物在计划密闭处置设施（CDF）在东芝加哥，印第安纳州，附近的两所学校。因此，该项目的重点是芝加哥空气中存在的挥发性较高的多氯联苯同源物，这些同源物在环境中具有很大的质量、毒性或持久性。植物可以从土壤和土壤-水中吸收多氯联苯同系物，将空气中的半挥发性同系物拦截在叶子和树皮的蜡质角质层上，并直接代谢污染物。此外，植物通过提供降解所需的生境、氧化还原电位和基质，刺激多氯联苯的根际生物修复。该项目的意义在于，通过进一步研究多氯联苯植物修复的基因组学、蛋白质组学和代谢组学基础，它将为制定和应用土地管理战略提供科学依据，以便对受污染的废物场地进行干预，并打破多氯联苯在大气中的连续循环以及随后对人类的暴露。项目5有四个具体目标： 使用GC/MS和LC/MS/MS确定选定PCB同系物（PCB-3、11、15、28、52、77、153）的植物代谢物和手性化合物（PCB-95、136）的吸收/分解/代谢 通过改变氧化还原条件使多氯联苯混合物矿化（在围隔生态系统和场地），在缺氧条件下使多氯联苯微生物脱氯，在有氧条件下使联苯环氧化 对选定的好氧PCB降解菌和已鉴定的暴露于PCB和PCB代谢物的厌氧降解菌的纯培养物进行蛋白质组学反应和毒性分析 <$利用T-RFLP分析和蛋白质组学分析，描述多氯联苯对CDF站点和沉积物土壤微生物群落造成的变化 竞争性更新的主要主题是更完整地确定PCB代谢物，生物转化蛋白质参与，并证明完全矿化的PCB同系物在植物根区的代谢组学和蛋白质组学的最新技术。

Project 5. Phytoremediation to Degrade Airborne PCB Congeners from Soil & Groundwater Sources The overall goal of Project 5 is to provide engineering research (non-biomedical) for the remediation of sites containing airborne PCB congeners which may expose humans. Specifically, it is to determine whether plants can provide In situ phytoremediation of PCB congeners from the air and other airborne sources like dredged sediments at the planned Confined Disposal Facility (CDF) in East Chicago, Indiana, near two schools. Thus the Project focuses on PCB congeners of higher volatility which are present in Chicago air and which display significant mass, toxicity or persistence in the environment. Plants can uptake PCB congeners from soil and soil-water, intercept semi-volatile congeners from the air onto the waxy cuticle of leaves and bark, and metabolize contaminants directly. In addition, plants stimulate rhizosphere bioremediaton of PCBs by providing the habitat, redox potential, and substrate necessary for degradation. The significance of this project is that, by studying further the genomic, proteomic, and metabolomic basis of PCB phytoremediation, it will provide the scientific basis for the development and application of land management strategies for intervention at contaminated waste sites, and to break the continuous cycling of PCBs in the atmosphere and subsequent exposure to humans. Four specific aims comprise Project 5: ¿ Identify plant metabolites of selected PCB congeners (PCB-3, 11, 15, 28, 52, 77, 153) and the uptake/resolution/metabolism of chiral compounds (PCB-95, 136) using GC/MS and LC/MS/MS ¿ Mineralize PCB mixtures (in mesocosms and site plots) by varying redox conditions which microbially dechlorinates PCBs under anoxic conditions and oxidizes the biphenyl ring under aerobic conditions ¿ Analyze the proteomic response and toxicity to pure cultures of selected aerobic PCB degraders and identified anaerobic degraders exposed to PCBs and PCB metabolites ¿ Characterize PCB-induced changes on the soil microbial community at CDF sites and sediments using T-RFLP analysis and proteomic analysis The leading themes of the competitive renewal are to identify more completely the PCB metabolites, the biotransformation proteins involved, and to demonstrate complete mineralization of PCB congeners in the root zone of plants by using the latest techniques of metabolomics and proteomics.