Project 5: Phytoremediation to Degrade Airborne PCB Congeners from Soil

项目 5：通过植物修复来降解土壤中空气中的多氯联苯同系物

• 批准号: 7936631
• 负责人: LARRY W ROBERTSON
• 金额: $ 19.13万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-12 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7936631
• 关键词: Aerobic; Air; Area; Bacteria; Bioremediations; Chicago; Development; Ecosystem; Engineering; Environment; Exposure to; Genomics; Goals; Habitats; Human; In Situ; Indiana; Intercept; Intervention; Left; Metabolic Biotransformation; Metabolism; Oxidation-Reduction; Plant Roots; Plants; Polychlorinated Biphenyls; Proteins; Proteomics; Refuse Disposal; Research; Residual state; Resolution; Schools; Site; Soil; Solutions; Source; Techniques; Testing; Tissues; Toxic effect; Translational Research; Water; base; dechlorination; diphenyl; exposed human population; metabolomics; microbial community; mineralization; planetary Atmosphere; remediation; response; uptake; wasting

Project 5. Phytoremediation to Degrade Airborne PCB Congeners from Soil & Groundwater Sources The overall goal of Project 5 is to provide engineering research (non-biomedical) for the remediation of sites containing airborne PCB congeners which may expose humans. Specifically, it is to determine whether plants can provide In situ phytoremediation of PCB congeners from the air and other airborne sources like dredged sediments at the planned Confined Disposal Facility (CDF) in East Chicago, Indiana, near two schools. Thus the Project focuses on PCB congeners of higher volatility which are present in Chicago air and which display significant mass, toxicity or persistence in the environment. Plants can uptake PCB congeners from soil and soil-water, intercept semi-volatile congeners from the air onto the waxy cuticle of leaves and bark, and metabolize contaminants directly. In addition, plants stimulate rhizosphere bioremediaton of PCBs by providing the habitat, redox potential, and substrate necessary for degradation. The significance of this project is that, by studying further the genomic, proteomic, and metabolomic basis of PCB phytoremediation, it will provide the scientific basis for the development and application of land management strategies for intervention at contaminated waste sites, and to break the continuous cycling of PCBs in the atmosphere and subsequent exposure to humans. Four specific aims comprise Project 5: ¿ Identify plant metabolites of selected PCB congeners (PCB-3, 11, 15, 28, 52, 77, 153) and the uptake/resolution/metabolism of chiral compounds (PCB-95, 136) using GC/MS and LC/MS/MS ¿ Mineralize PCB mixtures (in mesocosms and site plots) by varying redox conditions which microbially dechlorinates PCBs under anoxic conditions and oxidizes the biphenyl ring under aerobic conditions ¿ Analyze the proteomic response and toxicity to pure cultures of selected aerobic PCB degraders and identified anaerobic degraders exposed to PCBs and PCB metabolites ¿ Characterize PCB-induced changes on the soil microbial community at CDF sites and sediments using T-RFLP analysis and proteomic analysis The leading themes of the competitive renewal are to identify more completely the PCB metabolites, the biotransformation proteins involved, and to demonstrate complete mineralization of PCB congeners in the root zone of plants by using the latest techniques of metabolomics and proteomics.

项目5。植物修复从土壤和地下水中降低空气寄宿的PCB同源物的整体目标是提供工程研究（非生物医学），以修复含有可能暴露人类的空气生存PCB同源物的地点。具体而言，它是为了确定植物是否可以在空中和其他空气传播的来源和其他空气源来提供原位的植物修复，例如在印第安纳州东芝加哥的计划中的密闭处置设施（CDF），在印第安纳州东芝加哥，靠近两所学校。该项目的重点是芝加哥空气中存在的PCB较高波动性，并且在环境中表现出明显的质量，毒性或持久性。植物可以从土壤和土壤水中吸收PCB同源物，从空气中拦截半挥发物的同源物到叶子和树皮的蜡状切口，并直接代谢污染物。此外，植物通过提供降解所需的栖息地，氧化还原电位和底物来刺激PCB的根际生物体。该项目的意义在于，通过进一步研究PCB植物修复的基因组，蛋白质组学和代谢组基础，它将为在被污染的废物站点开发和应用土地管理策略的科学基础，并在大气和随后的暴露于丈夫的情况下破坏PCB的持续循环。四个特定目标包括项目5： »使用GC/MS和LC/MS/MS/MS/MS和LC/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS/MS »通过改变氧化还原条件的矿化PCB混合物（在中孔和现场图中），这些氧化还原条件会在缺氧条件下微生物脱氯并在有氧条件下氧化双苯基环 »分析对选定的有氧PCB降解器纯培养物的蛋白质组学反应和毒性，并鉴定出暴露于PCB和PCB代谢物的厌氧降解器 „使用T-RFLP分析和蛋白质组学分析表征PCB诱导的土壤微生物群落和沉积物的变化 竞争性更新的主要主题是通过使用最新的代谢组学和蛋白质组学的技术来更全面地确定PCB代谢产物，涉及的生物转化蛋白，并证明植物根部的PCB同源物的完全矿化。