CHARACTERIZATION OF MEMBRANE PROTEIN COMPLEXES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 中心，不一定是研究者的机构。 该项目的重点是开发和使用透射电子显微镜在表征膜蛋白-蛋白复合物。 我们将开发一个自动化的检测图像，选择和分类膜蛋白复合物的聚集状态，大小，构象状态和结构域的安排。一个目标是开发一个更系统的了解洗涤剂和脂质对膜蛋白及其复合物的稳定性和构象的影响，以帮助优化结晶的努力。 一个更雄心勃勃的目标是绘制出单个蛋白质结构域，以便更好地了解可能在构象或结构上异质的复合物中蛋白质之间的相互作用。 这种低分辨率信息可以与来自X射线和NMR研究的单个亚基或亚复合物的高分辨率模型相结合，以便更详细地了解复合物内的蛋白质-蛋白质相互作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project focuses on the development and use of transmission electron microscopy in characterizing membrane protein-protein complexes. We will develop an automated assay to image, select, and classify membrane protein complexes in terms of their aggregation state, size, conformational state and domain arrangement. One goal is to develop a more systematic understanding of the effect of detergents and lipids on the stability and conformation of membrane proteins and their complexes in order to help optimize crystallization efforts. A more ambitious goal is to map out individual protein domains in order to better understand the interactions between proteins in a complex that might be conformationally or structurally heterogeneous. This low resolution information can be combined with high-resolution models of individual subunits or subcomplexes, derived from X-ray and NMR studies, in order to develop a more detailed understanding of the protein-protein interactions within complexes.