CHARACTERIZATION OF MEMBRANE PROTEIN COMPLEXES

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 本项目的重点是发展和使用透射式电子显微镜来表征膜蛋白-蛋白质复合体。我们将开发一种自动化的分析方法，根据膜蛋白复合体的聚集状态、大小、构象状态和结构域排列来对其进行成像、选择和分类。一个目标是更系统地了解洗涤剂和脂类对膜蛋白及其复合体的稳定性和构象的影响，以帮助优化结晶努力。一个更雄心勃勃的目标是绘制出单个蛋白质结构域，以便更好地了解可能在构象或结构上不同的复合体中蛋白质之间的相互作用。这种低分辨率信息可以与来自X射线和核磁共振研究的单个亚基或亚复合体的高分辨率模型相结合，以便更详细地了解蛋白质-蛋白质在复合体中的相互作用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project focuses on the development and use of transmission electron microscopy in characterizing membrane protein-protein complexes. We will develop an automated assay to image, select, and classify membrane protein complexes in terms of their aggregation state, size, conformational state and domain arrangement. One goal is to develop a more systematic understanding of the effect of detergents and lipids on the stability and conformation of membrane proteins and their complexes in order to help optimize crystallization efforts. A more ambitious goal is to map out individual protein domains in order to better understand the interactions between proteins in a complex that might be conformationally or structurally heterogeneous. This low resolution information can be combined with high-resolution models of individual subunits or subcomplexes, derived from X-ray and NMR studies, in order to develop a more detailed understanding of the protein-protein interactions within complexes.