COMPARATIVE PROTEOMIC INVESTIGATION OF ARDS AND SYSTEMIC INFLAMMATORY INJURY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 急性呼吸窘迫综合征（ARDS）是重症监护医学中发病和死亡的重要原因之一。 它可能是许多疾病的后遗症，如机械通气，败血症和创伤。 该综合征的特征是炎症反应，导致肺泡-毛细血管屏障的破坏，导致液体和蛋白质从血液流入肺泡腔。 炎症反应的确切机制仍不完全清楚。 为了提高对这种疾病状态的理解和评估可能的治疗方案，已经进行了许多临床和实验性试验。 方法：拟议的研究重点是评价肺泡II型细胞从动物模型的ARDS相比，对照样品。 蛋白质标记后进行定量分析，通过层析、过滤和电泳分离，LC-MS-MS质谱法用于分析两组中的蛋白质组，以鉴定疾病的蛋白质标志物。 通过免疫测定进一步评价含量变化。 到目前为止，已经评估并发表了不同潮气量和肝缺血的机械通气后的变化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The acute respiratory distress syndrome (ARDS) is one of the most important causes for morbidity and mortality in intensive care medicine. It can be the sequel of numerous diseases like mechanical ventilation, sepsis and trauma. The syndrome is characterized by an inflammatory reaction that leads to a breakdown of the alveolar-capillary barrier, resulting in an influx of fluid and proteins from the blood into the alveolar space. The exact mechanism of the inflammatory reaction is still incompletely understood. Numerous clinical and experimental trials have been made in order to improve the understanding and evaluate possible treatment options of this disease state. METHODS: The proposed study focuses on the evaluation of alveloar type II cells from animal models of ARDS compared to control samples. After protein labeling for quantitative analysis, separation by chromatography, filtration and electrophoresis, LC-MS-MS mass spectrometry is used to analyze the proteome in both groups in order to identify protein markers of disease. Content changes are further evaluated by immunoassays. So far alterations after mechanical ventilation with different tidal volumes and liver ischemia have been evaluated and published.