COMPARATIVE PROTEOMIC INVESTIGATION OF ARDS AND SYSTEMIC INFLAMMATORY INJURY

ARDS 和全身炎症损伤的比较蛋白质组学研究

• 批准号: 7369046
• 负责人: MICHAEL A. MATTHAY
• 金额: $ 2.08万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7369046
• 关键词: COMPARATIVE; PROTEOMIC; INVESTIGATION; ARDS; SYSTEMIC

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The acute respiratory distress syndrome (ARDS) is one of the most important causes for morbidity and mortality in intensive care medicine. It can be the sequel of numerous diseases like sepsis, aspiration of gastric contents, pneumonia or major trauma. The syndrome is characterized by an inflammatory reaction that leads to a breakdown of the alveolar-capillary barrier, resulting in an influx of fluid and proteins from the blood into the alveolar space. The exact mechanism of the inflammatory reaction is still incompletely understood. Numerous clinical and experimental trials have been made in order to improve the understanding and evaluate possible treatment options of this disease state. METHODS: The proposed study focuses on the evaluation of pulmonary edema fluid samples of patients with ARDS compared to control samples from patients with cardiogenic pulmonary edema. After protein separation by chromatography, filtration and electrophoresis, mass spectrometry (MALDI-TOF-TOF, ESI) will be used to analyze the proteome and posttranslational modifications of proteins in both groups in order to identify protein markers of disease. Eventually, concentration changes shall be further evaluated by immunoassays. RESULTS: Using column chromatography and filtration, the pulmonary edema fluid samples were cleaned from debris and proteins could be separated by 1D SDS-PAGE. The results of MALDI-TOF-TOF are currently being evaluated. Analysis by High Pressure Liquid Chromatography and ESI is scheduled for next week.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心，不一定是研究者所在机构。急性呼吸窘迫综合征（ARDS）是重症监护医学中发病和死亡的重要原因之一。它可能是许多疾病的后遗症，如败血症，胃内容物吸入，肺炎或重大创伤。该综合征的特征是炎症反应，导致肺泡-毛细血管屏障的破坏，导致液体和蛋白质从血液流入肺泡腔。炎症反应的确切机制仍不完全清楚。为了提高对这种疾病状态的理解和评估可能的治疗方案，已经进行了许多临床和实验性试验。方法：拟议的研究重点是与心源性肺水肿患者的对照样本相比，评价ARDS患者的肺水肿液样本。通过色谱、过滤和电泳进行蛋白质分离后，将使用质谱法（MALDI-TOF-TOF，ESI）分析两组中蛋白质的蛋白质组和翻译后修饰，以鉴定疾病的蛋白质标志物。最终，应通过免疫测定进一步评价浓度变化。研究结果：使用柱层析和过滤，肺水肿液样品被从碎片中清除，并且蛋白质可以通过1D SDS-PAGE分离。目前正在评估MALDI-TOF-TOF的结果。通过高压液相色谱和ESI的分析定于下周进行。