COMPARATIVE PROTEOMIC INVESTIGATION OF ARDS AND SYSTEMIC INFLAMMATORY INJURY

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This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The acute respiratory distress syndrome (ARDS) is one of the most important causes for morbidity and mortality in intensive care medicine. It can be the sequel of numerous diseases like sepsis, aspiration of gastric contents, pneumonia or major trauma. The syndrome is characterized by an inflammatory reaction that leads to a breakdown of the alveolar-capillary barrier, resulting in an influx of fluid and proteins from the blood into the alveolar space. The exact mechanism of the inflammatory reaction is still incompletely understood. Numerous clinical and experimental trials have been made in order to improve the understanding and evaluate possible treatment options of this disease state. METHODS: The proposed study focuses on the evaluation of pulmonary edema fluid samples of patients with ARDS compared to control samples from patients with cardiogenic pulmonary edema. After protein separation by chromatography, filtration and electrophoresis, mass spectrometry (MALDI-TOF-TOF, ESI) will be used to analyze the proteome and posttranslational modifications of proteins in both groups in order to identify protein markers of disease. Eventually, concentration changes shall be further evaluated by immunoassays. RESULTS: Using column chromatography and filtration, the pulmonary edema fluid samples were cleaned from debris and proteins could be separated by 1D SDS-PAGE. The results of MALDI-TOF-TOF are currently being evaluated. Analysis by High Pressure Liquid Chromatography and ESI is scheduled for next week.