GLUTAMINE-DEPENDENT NAD+ SYNTHETASE FROM M TUBERCULOSIS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 谷氨酸依赖的NAD合成酶。结核病是一个重要的药物靶点。我们以前已经解决了这种酶的结构SAD。我们对两种不同类型络合物感兴趣。首先是不同的配体复合物的酶代表离散的稳定状态沿着反应坐标，这将有助于阐明的调节机制的活性在两个活性位点分开的40 A和连接的分子隧道。第二种类型的复合物是抑制剂复合物，对改善这种酶的抑制剂的设计至关重要。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Glutamine-dependent NAD synthetase from M. tuberculosis is an important drug target. We have previously solved the structure of this enzyme by SAD. We are interest in two different type of complexes. The first are different ligand complexes of the enzyme representing discrete stable states along the reaction coordinate that will contribute to the elucidation of the regulatory mechanism of the activity at two active sites separated by 40 A and connected by an molecular tunnel. The second type of complexes are inhibitor complexes crucial to improve the design of inhibitors of this enzyme.