HALIOTIS DIVERSICOLOR HEMOCYANIN

杂色鲍 血蓝蛋白

• 批准号: 8168590
• 负责人: Qing-Yu Zhang
• 金额: $ 2.15万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-15 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168590
• 关键词: AIDS Vaccines; Active Sites; Affinity; Allosteric Regulation; Amino Acid Sequence; Arthropods; Binding; Complex; Computer Retrieval of Information on Scientific Projects Database; Copper; Diagnostic; Funding; Grant; Haptens; Hemocyanin; Hemolymph; Human; Immune; Immunotherapeutic agent; Institution; Location; Malignant Neoplasms; Mammals; Maps; Morphology; Oxygen; Property; Reaction; Research; Research Personnel; Resources; Series; Source; Structure; United States National Institutes of Health; interest; macromolecular assembly; microbial; molecular assembly/self assembly; molecular mass; oxygen transport; polypeptide; respiratory protein; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The hemocyanins are copper-containing respiratory proteins often present as large molecular assemblies in the hemolymph of many mollusks and arthropods. Besides the function of transporting oxygen, hemocyanin can be an effective immune-stimulant in mammals (including humans). For example, the hemocyanin of the giant keyhole limpet Megathura crenulata finds application as an immunotherapeutic agent for the treatment of certain cancers (Helling et al., 1994; Helling et al., 1995), and is used as diagnostic tool for bilharziosis and as a hapten carrier for AIDS vaccine (Naylor et al., 1991). Another perspective is that hemocyanins are involved in immune reaction to microbial challenge of mollusks and arthropods (Destoumieux-Garzon et al., 2001). A number of molluscan and arthropod hemocyanins are being investigated in terms of morphology, amino acid sequence, subunit composition, and structure. The subunit of molluscan hemocyanins is a polypeptide of ~400 kDa, folded into a series of seven or eight globular substructures, the so-called 'functional units' (FUs). Such a FU has a molecular mass of 45-65 kDa and carries a single binuclear copper active site. Allosteric regulation and cooperativity of hemocyanin subunits (as in arthropods) or FUs (as in mollusks) are responsible for the high oxygen affinity of the whole hemocyanin macromolecular assembly. Zhang's lab is interested in the structure properties of whole hemocyanin bound with hapten as an immune-stimulant. Difference maps of the apo-hemocyanin and hemocyanin-hapten complex would provide the location of such binding

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 血红蛋白是含铜的呼吸蛋白，通常是许多软体动物和节肢动物的血淋巴中的大分子组件。除了运输氧气的功能外，血红蛋白还可以是哺乳动物（包括人）的有效免疫刺激性剂。例如，巨型钥匙孔的巨型巨型杂质的血红蛋白发现作为治疗某些癌症治疗的免疫治疗剂（Helling等，1994; Helling等，1995），并用作胆汁毛病的诊断工具，用于bilharziosis和hapten carser cahpten carterer（naylor）（naylor）。另一个观点是，血阳蛋白参与了对软体动物和节肢动物微生物挑战的免疫反应（Destoumieux-Garzon等，2001）。 正在研究许多软体动物和节肢动物血红蛋白蛋白，以形态学，氨基酸序列，亚基组成和结构。软体动物血红蛋白的亚基是约400 kDa的多肽，折叠成七个或八个球状亚结构，即所谓的“功能单位”（FUS）。这样的FU的分子质量为45-65 kDa，并带有单个双核铜活性位点。血红蛋白亚基（如节肢动物中）或FUS（如软体动物中）的变构调节和合作性负责整个血红蛋白大分子分子组装的高氧亲和力。 张的实验室对与触觉作为免疫刺激剂结合的整个血素蛋白的结构特性感兴趣。 Apo-毛氰酸蛋白酶和血氰酸蛋白酶络合物的差异图将提供这种结合的位置