HALIOTIS DIVERSICOLOR HEMOCYANIN

杂色鲍 血蓝蛋白

• 批准号: 8361099
• 负责人: Qing-Yu Zhang
• 金额: $ 2.45万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361099
• 关键词: AIDS Vaccines; Active Sites; Affinity; Allosteric Regulation; Amino Acid Sequence; Arthropods; Binding; Complex; Copper; Diagnostic; Electron Microscopy; Funding; Grant; Haptens; Hemocyanin; Hemolymph; Human; Immune; Immunotherapeutic agent; Location; Malignant Neoplasms; Mammals; Maps; Morphology; National Center for Research Resources; Oxygen; Principal Investigator; Property; Reaction; Research; Research Infrastructure; Resources; Series; Source; Structure; United States National Institutes of Health; cost; interest; macromolecular assembly; macromolecule; microbial; molecular assembly/self assembly; molecular mass; oxygen transport; polypeptide; respiratory protein; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The hemocyanins are copper-containing respiratory proteins often present as large molecular assemblies in the hemolymph of many mollusks and arthropods. Besides the function of transporting oxygen, hemocyanin can be an effective immune-stimulant in mammals (including humans). For example, the hemocyanin of the giant keyhole limpet Megathura crenulata finds application as an immunotherapeutic agent for the treatment of certain cancers (Helling et al., 1994; Helling et al., 1995), and is used as diagnostic tool for bilharziosis and as a hapten carrier for AIDS vaccine (Naylor et al., 1991). Another perspective is that hemocyanins are involved in immune reaction to microbial challenge of mollusks and arthropods (Destoumieux-Garzon et al., 2001). A number of molluscan and arthropod hemocyanins are being investigated in terms of morphology, amino acid sequence, subunit composition, and structure. The subunit of molluscan hemocyanins is a polypeptide of ~400 kDa, folded into a series of seven or eight globular substructures, the so-called 'functional units' (FUs). Such a FU has a molecular mass of 45-65 kDa and carries a single binuclear copper active site. Allosteric regulation and cooperativity of hemocyanin subunits (as in arthropods) or FUs (as in mollusks) are responsible for the high oxygen affinity of the whole hemocyanin macromolecular assembly. Zhang's lab is interested in the structure properties of whole hemocyanin bound with hapten as an immune-stimulant. Difference maps of the apo-hemocyanin and hemocyanin-hapten complex would provide the location of such binding

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 血红蛋白是含铜的呼吸蛋白通常是大的 许多软体动物和节肢动物的血淋巴中的分子组件。除了 氧运输的功能，血氰烷蛋白可以是有效的免疫刺激剂 哺乳动物（包括人类）。例如，巨型钥匙孔的血红蛋白 Megathura crenulata发现应用作为治疗的免疫治疗剂 某些癌症（Helling等，1994； Helling等，1995），被用作诊断工具 用于Bilharziois和作为艾滋病疫苗的抗抗果载体（Naylor等，1991）。其他 观点是，血氰酸参与对微生物挑战的免疫反应 软体动物和节肢动物（Destoumieux-Garzon等，2001）。 正在研究许多软体动物和节肢动物血胶蛋白 形态学，氨基酸序列，亚基组成和结构。亚基 软体动物血胶蛋白是〜400 kDa的多肽，折叠成七个或七个或 八个球状子结构，即所谓的“功能单元”（FUS）。这样的fu有一个 分子质量为45-65 kDa，并带有单个双核铜活性位点。变构 血红蛋白亚基（如节肢动物中）或FUS的调节和合作性（如 Mollusks）负责整个血红蛋白的高氧亲和力 大分子组件。 张的实验室对与整个血氰酸蛋白结合的结构特性感兴趣 触发为免疫刺激剂。 apo-Hemocyanin和 血素蛋白hapten复合物将提供这种结合的位置