PROCHLOROCOCCUS BACTERIA AND PHAGE INTERACTIONS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 地球大气中近一半的氧气是由海洋浮游植物产生的，即光养藻类和蓝藻。蓝藻，有时被错误地命名为蓝藻，是所有光合作用生物中最简单的，是进化成叶绿体的古老原共生生物的后代；因此，它们是研究光合作用的极好模型。这些微生物中数量最多的物种是微蓝细菌，原氯球菌Marinus。 在这项工作中，我们使用电子冷冻断层扫描来检测多个原氯球菌菌株(MED4，MIT-9313和Natl-1A)和感染它们的噬菌体。此外，我们还确定了感染细菌的分离噬菌体的结构。我们的目标是在感染过程中获得噬菌体高分辨率结构的快照。这将需要电子冷冻层析成像和单粒子平均相结合。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Nearly half of Earth's atmospheric oxygen is produced by marine phytoplankton, namely phototrophic algae and cyanobacteria. Cyanobacteria, sometimes misnamed blue-green algae, are the simplest of all photosynthetic organisms and are the descendents of the ancient proto-symbiotic organisms that evolved into chloroplasts; as such, they are an excellent model for studying photosynthesis. The most numerically abundant species of these microbes is the pico-cyanobacterium, Prochlorococcus marinus. In this work, we use electron cryotomography to examine multiple strains of Prochlorococcus (MED4, MIT-9313, and Natl-1A) and the bacteriophages that infect them. In addition, we also determine the structure of the isolated phages that infect the bacteria. We aim at obtaining a snap shots of high resolution structures of the phages during the infection process. This will require a combination of electron cryotomography and single particle averaging.