PROCHLOROCOCCUS BACTERIA AND PHAGE INTERACTIONS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 为子项目列出的总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 地球大气中近一半的氧气是由海洋浮游植物产生的， 光合藻类和蓝细菌。蓝细菌，有时被误称为蓝绿色 藻类，是所有光合生物中最简单的，是光合作用的后代。 进化成叶绿体的古老原共生生物;因此，它们是一种 是研究光合作用的绝佳模型 数量最丰富的物种 这些微生物是微微蓝细菌，海洋原绿球藻。 在这项工作中，我们使用电子冷冻断层扫描检查多株原绿球藻 (MED4、MIT-9313和Natl-1A）和感染它们的噬菌体。另外我们 还可以确定感染细菌的分离的病原体的结构。我们的目标是 在感染期间获得高分辨率结构的快照 过程这将需要结合电子冷冻断层扫描和单粒子 平均

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Nearly half of Earth's atmospheric oxygen is produced by marine phytoplankton, namely phototrophic algae and cyanobacteria. Cyanobacteria, sometimes misnamed blue-green algae, are the simplest of all photosynthetic organisms and are the descendents of the ancient proto-symbiotic organisms that evolved into chloroplasts; as such, they are an excellent model for studying photosynthesis. The most numerically abundant species of these microbes is the pico-cyanobacterium, Prochlorococcus marinus. In this work, we use electron cryotomography to examine multiple strains of Prochlorococcus (MED4, MIT-9313, and Natl-1A) and the bacteriophages that infect them. In addition, we also determine the structure of the isolated phages that infect the bacteria. We aim at obtaining a snap shots of high resolution structures of the phages during the infection process. This will require a combination of electron cryotomography and single particle averaging.