PNPASE COMPLEX

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 研究中心，而研究中心不一定是研究者所在的机构。 在人类基因组中存在一种易于识别的PNPase-like分子，其定位于线粒体，并且其缺陷导致线粒体功能障碍。重要的是，我们已经发现了重组SUV 3和PNP在体外的直接相互作用。我们计划进一步详细研究它们的相互作用，以测试SUV 3是否与PNTR在监测线粒体RNA降解方面协同工作。在降解体中是否有其他蛋白质与SUV 3/PNTR一起存在？如果是，它们在RNA降解中的作用是什么？从线粒体中分离和鉴定SUV 3/PNTR复合物是推进我们目前对哺乳动物线粒体降解体的认识的关键步骤。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. There is a readily recognizable PNPase-like molecule in the human genome, which localizes to mitochondria, and its deficiency leads to mitochondrial dysfunction. Importantly, we have found a direct interaction between recombinant SUV3 and PNPase in vitro. We plan to further examine their interaction in detail to test whether SUV3 works in concert with PNPase in monitoring mitochondrial RNA degradation. Are there other proteins in the degradosome together with SUV3/PNPase? If yes, what are their roles in RNA degradation? Isolating and identifying the SUV3/PNPase complex from mitochondria are critical steps to advance our current knowledge of the mammalian mitochondrial degradosome.