PNPASE COMPLEX

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 在人类基因组中有一种很容易识别的PNPase样分子，它定位于线粒体，它的缺失导致线粒体功能障碍。重要的是，我们在体外发现了重组SUV3与PNPase之间的直接相互作用。我们计划进一步详细研究它们的相互作用，以测试SUV3是否与PNPase在监控线粒体RNA降解方面协同工作。除SUV3/PNPase外，降解体中还存在其他蛋白质吗？如果是，它们在RNA降解中起什么作用？从线粒体中分离和鉴定SUV3/PNPase复合体是提高我们对哺乳动物线粒体降解体现有知识的关键步骤。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. There is a readily recognizable PNPase-like molecule in the human genome, which localizes to mitochondria, and its deficiency leads to mitochondrial dysfunction. Importantly, we have found a direct interaction between recombinant SUV3 and PNPase in vitro. We plan to further examine their interaction in detail to test whether SUV3 works in concert with PNPase in monitoring mitochondrial RNA degradation. Are there other proteins in the degradosome together with SUV3/PNPase? If yes, what are their roles in RNA degradation? Isolating and identifying the SUV3/PNPase complex from mitochondria are critical steps to advance our current knowledge of the mammalian mitochondrial degradosome.