LOW-POWER KA BAND BRIDGE FOR PULSED ENDOR APPLICATION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 除了自旋标记蛋白质的折叠，斯科尔斯实验室的主要基础研究领域是Endor(电子-核双共振)在顺磁性金属蛋白中的应用。到目前为止，斯科尔斯一直使用CW Endor方法。脉冲Endor与CW Endor相比，将为报告金属-配体结构的小耦合提供更好的分辨率。脉冲微波的技术复杂性，加上商业设备约50万美元的费用，一直是斯科尔斯实验室不做脉冲Endor的原因。Peter Borbat在Freed实验室的最新开发表明，在Acert上可以以~50,000美元的价格制作具有中等脉冲输出(1至5 W)、适当的定时电路、相移、脉冲编程和数据采集的脉冲Q频带电桥。能够进行脉冲Endor将是斯科尔斯实验室的一项重大技术进步。奥尔巴尼和康奈尔的临近将使信息和知识的转移变得容易。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Besides the folding of spin labeled proteins, the major fundamental research area of the Scholes lab is the application of ENDOR (electron-nuclear double resonance) to paramagnetic metalloproteins. Until now Scholes has used CW ENDOR methods. Pulsed ENDOR as opposed to CW ENDOR, would provide better resolution of small couplings that report on metal-ligand structure. The technical complexity of pulsed microwaves together with the ~$500,000 expense of commercial equipment have been the reasons why the Scholes lab does not do pulsed ENDOR. Recent developments of Peter Borbat in the Freed lab have made it clear that a pulsed Q-band bridge having moderate pulse output (1 to 5 W), appropriate timing circuits, phase shifting, pulse programming, and data collection can be made at ACERT for ~$50,000. The ability to do pulsed ENDOR will be a major technical advancement for the Scholes lab. The proximity of Albany and Cornell will make for easy transfer of information and knowledge.