OLIGOMERIZATION STATE OF THE MONOAMINE OXIDASES

单胺氧化酶的低聚状态

• 批准号: 8363971
• 负责人: PETER P BORBAT
• 金额: $ 0.05万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363971
• 关键词: Active Sites; Amines; Binding; Data; Enzymes; Funding; Grant; Human; Measurement; Membrane; Monoamine Oxidase; Monoamine Oxidase A; Mutation; National Center for Research Resources; Outer Mitochondrial Membrane; Pargyline; Play; Positioning Attribute; Principal Investigator; Rattus; Research; Research Infrastructure; Resources; Role; Sampling; Source; Spin Labels; Structure; Study models; Technology; Testing; United States National Institutes of Health; analog; cost; inhibitor/antagonist; mutant; neurotransmitter metabolism; quantum

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Monoamine oxidases ( A & B) are outer mitochondrial membrane-bound enzymes that play important roles in amine neurotransmitter metabolism. Sequence and modeling studies suggest human MAO A differs from all other mammalian MAO A in that it is monomeric rather than dimeric in the outer membrane due to a Glu151Lys mutation. Crystal structures of purified human and rat MAO A provide additional support for this hypothesis. To provide a direct experimental test, analogues of the inhibitor pargyline with a nitroxide spin label on the para position will be synthesized and covalently incorporated into the active sites of human and rat MAO A, the Lys151Glu mutant of human MAO A, and human MAO B. Structural data of the dimeric form of crystalline human MAO B show the distance between the active site cavities is 42 ¿. This distance is readily determined by Double Quantum Coherence EPR measurements on these samples. The dipolar spectral data will provide distance information that will determine if these enzyme species are in their dimeric or monomeric forms in the mitochondrial outer membrane.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 单胺氧化酶（A & B）是线粒体外膜结合酶，在胺类神经递质代谢中起重要作用。 序列和建模研究表明，人类单胺氧化酶A不同于所有其他哺乳动物单胺氧化酶A，因为它是单体，而不是二聚体的外膜由于Glu 151 Lys突变。 纯化的人和大鼠单胺氧化酶A的晶体结构为这一假设提供了额外的支持。 为了提供直接的实验测试，将合成在帕拉上具有氮氧自旋标记的抑制剂帕吉林的类似物，并将其共价结合到人和大鼠MAO A、人MAO A的Lys 151 Glu突变体和人MAO B的活性位点中。 晶体人类单胺氧化酶B的二聚体形式的结构数据显示活性位点空腔之间的距离为42。这个距离很容易通过对这些样品的双量子相干EPR测量来确定。 偶极光谱数据将提供距离信息，其将确定这些酶种类在线粒体外膜中是以其二聚体形式还是单体形式。