STRUCTURAL STUDIES OF METHYLTRANSFERASE MHAE III BOUND TO SUBSTRATE DNA

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 该项目需要详细研究甲基转移酶与底物DNA的相互作用。虽然DNA胞嘧啶-5甲基转移酶催化的细节目前已经非常详细，但在化学反应发生之前发生的构象变化尚未被描述。DNA胞嘧啶-5甲基转移酶靶向特定胞嘧啶进行挤出和随后甲基化的机制尚不清楚。因此，我们的目标是在催化作用发生之前获得与DNA结合的M.HaeIII早期中间体的结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project entails a detailed study of the interaction of the methyltransferases with the substrate DNA. Though the details of catalysis by DNA cytosine-5 methyltransferases are currently known in great detail, the conformational changes that happen before the chemistry takes place have not yet been described. The mechanism by which DNA cytosine-5 methyltransferases target a particular cytosine for extrusion and later methylation is unknown. Hence our goal is to obtain structures of early intermediates of M.HaeIII bound to DNA before the catalysis occurs.