STRUCTURAL STUDIES OF METHYLTRANSFERASE MHAE III BOUND TO SUBSTRATE DNA

甲基转移酶 MHAE III 与底物 DNA 结合的结构研究

• 批准号: 8169327
• 负责人: GREGORY Lawrence VERDINE
• 金额: $ 0.35万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169327
• 关键词: Binding; Catalysis; Chemistry; Computer Retrieval of Information on Scientific Projects Database; Cytosine; DNA; Funding; Goals; Grant; Institution; Methylation; Methyltransferase; Research; Research Personnel; Resources; Source; Structure; United States National Institutes of Health

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This project entails a detailed study of the interaction of the methyltransferases with the substrate DNA. Though the details of catalysis by DNA cytosine-5 methyltransferases are currently known in great detail, the conformational changes that happen before the chemistry takes place have not yet been described. The mechanism by which DNA cytosine-5 methyltransferases target a particular cytosine for extrusion and later methylation is unknown. Hence our goal is to obtain structures of early intermediates of M.HaeIII bound to DNA before the catalysis occurs.

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