DISULPHIDE CROSS-LINKED RARE SEARCH INTERMEDIATE OF HOGG1 ON UNDAMAGED DNA

未受损 DNA 上 HOGG1 的二硫键交联稀有搜索中间体

基本信息

  • 批准号:
    8169232
  • 负责人:
  • 金额:
    $ 0.35万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2010
  • 资助国家:
    美国
  • 起止时间:
    2010-04-01 至 2011-03-31
  • 项目状态:
    已结题

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The mechanism by which DNA glycosylases differentiate between specific damaged bases and the overwhelming excess of normal bases remains a mystery. Moreover, it is also unclear how these enzymes, despite spending so much time interrogating normal DNA, constrain themselves to cleaving only damaged bases. In particular, the feat of recognizing 8-oxoguanine lesions (oxoG) is impressive, because guanine and oxoG differ by merely two atoms, and the presence of oxoG in B-form DNA elicits no significant structural perturbation. Per our efforts to better understand these issues, we have used disulfide cross-linking technology to covalently trap a human 8-oxoguanine glycosylase (hOGG1) to an undamaged G:C base pair in a normal duplex of DNA. The x-ray structure of this complex reveals that the target guanine base has been swiveled out from the base stack and is fully inserted into the extrahelical active site pocket. Interestingly, though the enzyme is catalytically active, the target guanine remains uncleaved, an observation supported by biochemical analysis. The fact that even complete binding of guanine in the active site does not result in base cleavage demonstrates the existence of discrimination between G and oxoG at the level of catalysis.
这个子项目是众多研究子项目之一

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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GREGORY Lawrence VERDINE其他文献

GREGORY Lawrence VERDINE的其他文献

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{{ truncateString('GREGORY Lawrence VERDINE', 18)}}的其他基金

DISULPHIDE CROSS-LINKED RARE SEARCH INTERMEDIATE OF HOGG1 ON UNDAMAGED DNA
未受损 DNA 上 HOGG1 的二硫键交联稀有搜索中间体
  • 批准号:
    8361617
  • 财政年份:
    2011
  • 资助金额:
    $ 0.35万
  • 项目类别:
STRUCTURAL STUDIES OF METHYLTRANSFERASE MHAE III BOUND TO SUBSTRATE DNA
甲基转移酶 MHAE III 与底物 DNA 结合的结构研究
  • 批准号:
    8361671
  • 财政年份:
    2011
  • 资助金额:
    $ 0.35万
  • 项目类别:
DNA SEARCH AND BASE FLIPPING MECHANISMS OF DNA GLYCOSYLASE, MUTM
DNA 糖基化酶 MUTM 的 DNA 搜索和碱基翻转机制
  • 批准号:
    8361618
  • 财政年份:
    2011
  • 资助金额:
    $ 0.35万
  • 项目类别:
STRUCTURAL STUDIES OF NUCLEOTIDE EXCISION REPAIR ENZYMES
核苷酸切除修复酶的结构研究
  • 批准号:
    8361603
  • 财政年份:
    2011
  • 资助金额:
    $ 0.35万
  • 项目类别:
UNDERSTANDING THE BROAD SUBSTRATE SPECIFICITY OF ALKA AT THE ATOMIC LEVEL
在原子水平上了解 ALKA 的广泛底物特异性
  • 批准号:
    8361670
  • 财政年份:
    2011
  • 资助金额:
    $ 0.35万
  • 项目类别:
STRUCTURAL STUDIES OF NUCLEOTIDE EXCISION REPAIR ENZYMES
核苷酸切除修复酶的结构研究
  • 批准号:
    8169208
  • 财政年份:
    2010
  • 资助金额:
    $ 0.35万
  • 项目类别:
DNA SEARCH AND BASE FLIPPING MECHANISMS OF DNA GLYCOSYLASE, MUTM
DNA 糖基化酶 MUTM 的 DNA 搜索和碱基翻转机制
  • 批准号:
    8169233
  • 财政年份:
    2010
  • 资助金额:
    $ 0.35万
  • 项目类别:
UNDERSTANDING THE BROAD SUBSTRATE SPECIFICITY OF ALKA AT THE ATOMIC LEVEL
在原子水平上了解 ALKA 的广泛底物特异性
  • 批准号:
    8169326
  • 财政年份:
    2010
  • 资助金额:
    $ 0.35万
  • 项目类别:
STRUCTURAL STUDIES OF AN ADENINE DNA GLYCOSYLASE, MUTY
腺嘌呤 DNA 糖基化酶 MUTY 的结构研究
  • 批准号:
    8169328
  • 财政年份:
    2010
  • 资助金额:
    $ 0.35万
  • 项目类别:
STRUCTURAL STUDIES OF METHYLTRANSFERASE MHAE III BOUND TO SUBSTRATE DNA
甲基转移酶 MHAE III 与底物 DNA 结合的结构研究
  • 批准号:
    8169327
  • 财政年份:
    2010
  • 资助金额:
    $ 0.35万
  • 项目类别:

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