STRUCTURAL STUDIES OF METHYLTRANSFERASE MHAE III BOUND TO SUBSTRATE DNA

甲基转移酶 MHAE III 与底物 DNA 结合的结构研究

• 批准号: 8361671
• 负责人: GREGORY Lawrence VERDINE
• 金额: $ 0.29万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361671
• 关键词: Binding; Catalysis; Chemistry; Cytosine; DNA; Funding; Goals; Grant; Methylation; Methyltransferase; National Center for Research Resources; Principal Investigator; Research; Research Infrastructure; Resources; Source; Structure; United States National Institutes of Health; cost; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. DNA. Though the details of catalysis by DNA cytosine-5 methyltransferases are currently known in great detail, the conformational changes that happen before the chemistry takes place have not yet been described. The mechanism by which DNA cytosine-5 methyltransferases target a particular cytosine for extrusion and later methylation is unknown. Hence our goal is to obtain structures of early intermediates of M.HaeIII bound to DNA before the catalysis occurs.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 DNA.虽然DNA胞嘧啶-5甲基转移酶催化的细节目前已经非常详细，但在化学反应发生之前发生的构象变化尚未被描述。DNA胞嘧啶-5甲基转移酶靶向特定胞嘧啶进行挤出和随后甲基化的机制尚不清楚。因此，我们的目标是在催化作用发生之前获得与DNA结合的M.HaeIII早期中间体的结构。