DROSOPHILA TORC ASSOCIATED PROTEINS

果蝇 Torc 相关蛋白

• 批准号: 8171243
• 负责人: MARC R MONTMINY
• 金额: $ 0.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171243
• 关键词: CREB1 gene; Cell Nucleus; Computer Retrieval of Information on Scientific Projects Database; Cues; Cyclic AMP; Cytoplasm; Drosophila genus; Equilibrium; Family; Fasting; Funding; Gene Expression; Genes; Genetic; Glucagon; Glucose; Grant; Hormonal; Institution; Intracellular Second Messenger; Mammals; Mediating; Pancreas; Proteins; Proteomics; Research; Research Personnel; Resources; Role; Second Messenger Systems; Signal Transduction; Source; Starvation; United States National Institutes of Health; energy balance; feeding; insight; programs; promoter; response; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The intracellular second messenger cAMP functions as a starvation state signal, which mediates induction of catabolic programs in response to hormonal stimulation. Glucose balance in fasted mammals is maintained, for example, by induction of gluconeogenic genes by the cAMP responsive CREB coactivator TORC2 in response to elevations in circulating pancreatic glucagon. Sequestered in the cytoplasm under feeding conditions, TORC2 is dephosphorylated and translocated to the nucleus in response to cAMP signaling, whereupon it stimulates gene expression by associating with CREB over relevant promoters. To further dissect the role of the TORC family in energy balance, we will use proteomic and genetic tools to manipulate TORC activity in Drosophila. The identification of Drosophila TORC-associated proteins will provide new insights into the mechanisms by which this coactivator responds to environmental cues.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 细胞内第二信使 cAMP 作为饥饿状态信号，介导响应激素刺激的分解代谢程序的诱导。 例如，通过 cAMP 响应性 CREB ​​共激活因子 TORC2 响应循环胰腺胰高血糖素的升高而诱导糖异生基因，从而维持禁食哺乳动物中的葡萄糖平衡。 在喂养条件下，TORC2 被隔离在细胞质中，响应 cAMP 信号传导，TORC2 被去磷酸化并易位到细胞核，随后它通过相关启动子与 CREB ​​结合来刺激基因表达。为了进一步剖析 TORC 家族在能量平衡中的作用，我们将使用蛋白质组学和遗传工具来操纵果蝇中的 TORC 活性。 果蝇 TORC 相关蛋白的鉴定将为该共激活因子响应环境线索的机制提供新的见解。