PHOSPHORYLATION OF MAD1 BY TTK

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 细胞周期的进程在多个检查点进行调节。例如，纺锤体检查点监测染色体分离的保真度。这个检查点阻止细胞进入后期，直到所有姐妹染色单体都正确地附着在有丝分裂纺锤体上并排列在中期板上。已证明许多蛋白质在这一过程中起重要作用，包括MAD1、MAD2、TTK、Bub1、BubR1和BubR2。为了进一步了解这些蛋白调节纺锤体检查点的分子机制，我们进行了质谱分析，以确定TTK激酶在Mad1上的磷酸化位点。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Progression through the cell cycle is regulated at multiple checkpoints. For example, the spindle checkpoint monitors the fidelity of chromosome segregation. This checkpoint inhibits cells from progressing into anaphase until all sister chromatids are properly attached to the mitotic spindle and aligned at the metaphase plate. A number of proteins have been shown to be important in this process including MAD1, MAD2, TTK, Bub1, BubR1 and BubR2. To further understand the molecular mechanism by which these protein regulated the spindle checkpoint a mass spectrometry analysis was performed to identify phosphorylation sites on Mad1 by the TTK kinase.