PHOSPHORYLATION OF MAD1 BY TTK

TTK 磷酸化 MAD1

• 批准号: 8171354
• 负责人: Don W Cleveland
• 金额: $ 0.24万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171354
• 关键词: Anaphase; Cell Cycle; Cells; Chromosome Segregation; Computer Retrieval of Information on Scientific Projects Database; Funding; Grant; Institution; MXI1 gene; Mass Spectrum Analysis; Metaphase Plate; Mitotic spindle; Molecular; Monitor; Phosphorylation; Phosphorylation Site; Phosphotransferases; Process; Proteins; Research; Research Personnel; Resources; Sister Chromatid; Source; United States National Institutes of Health; numb protein

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Progression through the cell cycle is regulated at multiple checkpoints. For example, the spindle checkpoint monitors the fidelity of chromosome segregation. This checkpoint inhibits cells from progressing into anaphase until all sister chromatids are properly attached to the mitotic spindle and aligned at the metaphase plate. A number of proteins have been shown to be important in this process including MAD1, MAD2, TTK, Bub1, BubR1 and BubR2. To further understand the molecular mechanism by which these protein regulated the spindle checkpoint a mass spectrometry analysis was performed to identify phosphorylation sites on Mad1 by the TTK kinase.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 细胞周期的进程在多个检查点受到调节。 例如，纺锤体检查点监测染色体分离的保真度。 这个检查点抑制细胞进入后期，直到所有的姐妹染色单体都正确地附着在有丝分裂纺锤体上并在中期板上对齐。 许多蛋白质已被证明在这个过程中是重要的，包括MAD 1，MAD 2，TTK，Bub 1，BubR 1和BubR 2。 为了进一步了解这些蛋白质调节纺锤体检查点的分子机制，进行质谱分析以鉴定TTK激酶对Mad 1的磷酸化位点。