STRUCTURAL STUDIES OF RIBOSWITCHES

核开关的结构研究

• 批准号: 8170651
• 负责人: ALEXANDER A SERGANOV
• 金额: $ 0.71万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170651
• 关键词: Affect; Amino Acids; Bacteria; Binding; Coenzymes; Computer Retrieval of Information on Scientific Projects Database; Elements; Eukaryota; Funding; Gene Expression; Gene Targeting; Genes; Grant; Institution; Laboratories; Ligand Binding Domain; Prokaryotic Cells; Proteins; RNA; Regulation; Research; Research Personnel; Resources; Source; Structure; Translations; United States National Institutes of Health; conformational conversion; improved; sugar

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Expression of many genes in bacteria can be controlled by metabolite molecules without initial protein participation. However, mechanism of such regulation was not clear for many years. Recently several laboratories discovered that small metabolites directly interact with the 5 regions of mRNAs, modulate RNA structures and affect expression of the target genes. Such new type of gene expression control is now described for many genes in prokaryotes and some genes in eukaryotes and is associated with binding of coenzymes, nucleobases, aminoacids, and sugars. The metabolite-sensing RNA regions, termed riboswitches, are composed of a ligand-binding domain and expression platform that contains RNA elements involved in gene expression. The conformational transitions associated with metabolite binding are then interpreted through the expression platforms leading to regulation on the levels of either translation or transcription.Our research efforts are focused on the structures of the metabolite-sensing modules in the free and bound states, towards an improved understanding of the conformational transitions harnessed by the expression platforms for allosteric modulation of gene expression.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 细菌中许多基因的表达可以在没有初始蛋白质参与的情况下由代谢物分子控制。然而，多年来，这种调节的机制并不清楚。近年来，一些实验室发现小分子代谢物直接与mRNA的5个区域相互作用，调节RNA结构，影响靶基因的表达。这种新型的基因表达控制现在被描述用于原核生物中的许多基因和真核生物中的一些基因，并且与辅酶、核碱基、氨基酸和糖的结合有关。被称为核糖开关的代谢物传感RNA区域由配体结合结构域和表达平台组成，所述表达平台包含参与基因表达的RNA元件。我们的研究工作集中在代谢物传感模块在自由和结合状态下的结构上，旨在更好地理解表达平台对基因表达变构调控的构象转换。