MULTI CRYSTAL EXPERIMENTS WITH INSULIN

胰岛素多晶体实验

• 批准号: 8170628
• 负责人: ALEXEI SUAREZ SOARES
• 金额: $ 1.34万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170628
• 关键词: Acoustics; Computer Retrieval of Information on Scientific Projects Database; Crystallography; Funding; Grant; Hand; Institution; Insulin; Muramidase; Pattern; Preparation; Research; Research Personnel; Resources; Source; Specimen; Structure; Testing; United States National Institutes of Health; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Acoustic droplet ejection (ADE) was used to transfer 2.5uL droplets of crystal slurries from 396 well plates onto MiteGen micro mesh pins. Multiple homogenous slurries will be tested to verify that acoustic specimen preparation is effective and non-damaging for R3 insulin and P43212 lysozyme crystals of different sizes, different concentrations, and different overall purity and cleanliness. All tested conditions yielded accurately transferred droplets of precise volumes. We will show that transferred droplets yield undamaged and well diffracting crystals, with diffraction patterns and the resulting structures comparable for ADE mounted and hand mounted specimens.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 使用声学液滴喷射（ADE）将2.5uL晶体浆料液滴从396孔板转移到MiteGen微网针上。 将对多个均质浆液进行测试，以验证声学样本制备对于不同尺寸、不同浓度和不同总体纯度和清洁度的R3胰岛素和P43212溶菌酶晶体是有效且无损伤的。 所有测试条件均产生精确体积的准确转移液滴。 我们将表明，转移液滴产生未损坏和衍射晶体，衍射图案和所得结构可比的ADE安装和手工安装的标本。