HIGH PRESSURE EXPERIMENTS WITH CUBIC INSULIN AND RHOMBOHEDRAL INSULIN

立方胰岛素和菱面体胰岛素的高压实验

• 批准号: 7358946
• 负责人: ALEXEI SUAREZ SOARES
• 金额: $ 0.45万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7358946
• 关键词: PRESSURE; EXPERIMENTS; CUBIC; INSULIN; RHOMBOHEDRAL

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are determining the effectiveness of using high pressure freezing of crystals to provide cryo protection, without the need for conventional chemical cryo protection. This evaluation will be useful in deciding how we with to proceed with respect to transfering this technology to the PxRR and the NSLS for use by the general user community. We are working in collaboration with the group at CHESS that developed this technology, and with the NSLS. Crystals of cubic insulin and rhombohedral insulin were prepared and frozen under vaying conditions of pressure and cryo protectant concentration. This method has previously proven most useful in crystals with relatively low solvent content, and the very large difference in the solvent content of the two specimen types (30% for rhobohedral insulin and 70% for cubic insulin) should provide data on this trend. A secondary objective of this project is to evaluate the density of the amorphous ice in the solvent region. The 011 reflection of cubic insulin is highly sensitive to the density contrast in the specimen, and we will track its intensity as the pressure is raised.

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构适用于该中心，这不一定是调查员的机构。我们正在确定使用高压冻结晶体提供冷冻保护的有效性，而无需传统的化学冷冻保护。这项评估对于决定如何将这项技术转移到PXRR和NSLS供普通用户社区使用的NSL将是有用的。我们正在与国际象棋的小组合作，该小组与NSL一起开发了这项技术。在压力和冷冻保护剂浓度的瓦伊条件下制备了立方胰岛素和菱形胰岛素的晶体。此方法先前已证明在溶剂含量相对较低的晶体中最有用，两种样品类型的溶剂含量的差异很大（对于若贝德中胰岛素的30％，三次胰岛素的70％）应提供有关此趋势的数据。 该项目的次要目标是评估溶剂区域中无定形冰的密度。立方胰岛素的011反射对样品的密度对比度高度敏感，我们将随着压力的升高跟踪其强度。