NITROSYL HORSE HEART MYOGLOBIN

亚硝基马心肌红蛋白

• 批准号: 8170624
• 负责人: George Bannerman Richter-Addo
• 金额: $ 0.46万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8170624
• 关键词: Binding; Computer Retrieval of Information on Scientific Projects Database; Crystallography; Cytochrome P450; Data; Data Collection; Deposition; Equus caballus; Funding; Gases; Grant; Heart; Hemeproteins; Hemoglobin; Housing; Inorganic Chemistry; Institution; Journals; Knowledge; Link; Manuscripts; Metals; Methods; Molecular Conformation; Myoglobin; Nitric Oxide; Nitrites; Positioning Attribute; Preparation; Publishing; Research; Research Personnel; Resolution; Resources; Signaling Molecule; Source; Structure; Time; Torsion; United States National Institutes of Health; Work

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Nitric oxide (NO) is an important signaling molecule, and it binds to the metal center in heme proteins such as myoglobin (Mb), hemoglobin, and cytochrome P450. The inorganic chemistry of the Fe-NO link is fairly well developed, however, the Fe-NO units in heme proteins are sometimes not very well defined in terms of their structural conformation and thus reactivity. We have recently published the 1.9 ang structure of horse heart MbNO (in 2003). During subsequent experimental work, we found that there were differences in the observed Fe-N-O angle in MbNO depending on how the compound is made (whether from nitrite (NO2) or from NO gas). The compound formed from nitrite gives reproducible FeNO angles of 145 deg, and the compound formed from NO gas gives reproducible angles of about 117 deg. To the best of our knowledge, this is the first time that such an observation has been made: that the Fe-NO geometry is dependent on the method of preparation. We thus propose to obtain higher resolution data to better determine the positions of the Fe, N, and O atoms of the FeNO moiety to more accurately determine the differences in the geometries (bond angles, distances, torsion angles, etc). SAMPLES: We will send a set of four crystals for the first method (NO gas method) and a set of six crystals for the alternate method (nitrite method). All crystals have been screened in-house. If possible, we would like data collection on all the crystals. This work will be published in a high-quality journal, and all coordinates deposited at the PDB at the time of manuscript submission.

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 一氧化氮（NO）是一种重要的信号分子，它与血红素蛋白（如肌红蛋白（Mb）、血红蛋白和细胞色素P450）中的金属中心结合。 Fe-NO连接的无机化学相当发达，然而，血红素蛋白中的Fe-NO单元有时在其结构构象和反应性方面不是很好地定义。 我们最近发表了马心MbNO的1.9ang结构（2003年）。 在随后的实验工作中，我们发现在MbNO中观察到的Fe-N-O角存在差异，这取决于该化合物是如何制成的（无论是从亚硝酸盐（NO2）还是从NO气体）。 由亚硝酸盐形成的化合物给出145度的可再现FeNO角，并且由NO气体形成的化合物给出约117度的可再现角。 据我们所知，这是第一次观察到这样的结果：Fe-NO几何形状取决于制备方法。 因此，我们建议获得更高的分辨率数据，以更好地确定FeNO部分的Fe，N和O原子的位置，以更准确地确定几何形状（键角，距离，扭转角等）的差异。 样品：我们将为第一种方法（NO气体法）发送一组四个晶体，为替代方法（亚硝酸盐法）发送一组六个晶体。 所有晶体均已在内部进行筛选。 如果可能的话，我们希望收集所有晶体的数据。 这项工作将发表在一个高质量的杂志上，所有的坐标在提交手稿时存放在PDB。