ANALYSIS OF COLD WAR C14 LEVELS IN DNA FROM HUMAN PROSTATE TISSUES

人类前列腺组织 DNA 中冷战时期 C14 水平的分析

• 批准号: 8171696
• 负责人: Donald James Vander Griend
• 金额: $ 1.7万
• 依托单位: UNIVERSITY OF CALIF-LAWRNC LVRMR NAT LAB
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171696
• 关键词: American; Archives; Benign Prostatic Hypertrophy; Biology; C14 isotope; Carbon; Cell physiology; Cells; Computer Retrieval of Information on Scientific Projects Database; DNA; Data; Date of birth; Development; Disease; Etiology; Funding; Generations; Goals; Grant; Growth; Health; Human; Individual; Institution; Life; Longevity; Maintenance; Malignant neoplasm of prostate; Measurement; Measures; Methods; Patients; Prostate; Prostatic; Prostatic Diseases; Quality Control; Reporting; Research; Research Personnel; Resources; Source; Specimen; Staging; Stem cells; Testing; Time; Tissue Extracts; Tissues; United States National Institutes of Health; War; anticancer research; cell type; male; men

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Introduction: Prostate diseases such as prostate cancer and prostatic enlargement (i.e. benign prostatic hyperplasia, or BPH) are major health problems among American men. The growth of the prostate gland occurs in various stages throughout the lifespan of male individuals, and both prostate cancer and BPH are the result of aberrant cellular turnover and growth. However, this process of cellular turnover is very poorly understood, particularly in humans. The goal of this project is to use AMS to measure Carbon-14 levels in the DNA of prostate cells as a result of Cold War atomic bomb testing. Measurement of Carbon-14 in the post-bomb testing era allows an accurate assessment of tissue turnover and cellular birth-dates; such an approach allows important questions in prostate biology research to be answered regarding the etiology of prostate disease. Methods: DNA will be extracted from tissue specimens, quality controlled, and submitted for Carbon-14 analyses using AMS. Tissue specimens will included archived tissue specimens from our institution (1955  present), as well as fresh prostate tissue specimens from patients currently at our institution. Carbon-14/Carbon-12 ratios will be compared to the known atmospheric Carbon-14 levels, as previously reported, and tissue and cellular "dates of birth" will be determined. Anticipated Results: We anticipate that AMS will accurately determine the rate of turnover within the prostate cellular compartments, and that such Carbon-14 levels will indicate the time of disease development when compared between normal prostate, prostate cancers, and BPH. Furthermore, such an approach will document the average lifespan of the various cell types within the prostate gland, such as the long-lived prostate stem cell. Finally, data generated from these studies will greatly facilitate the generation of new hypotheses regarding prostate function and disease etiology. Impact: Data generated from these studies will have an immediate impact on the field of prostate cancer research and will facilitate answers to essential - and as yet unanswered - questions pertaining to prostate growth, maintenance, and the etiology of prostate disease. Introduction: Prostate diseases such as prostate cancer and prostatic enlargement (i.e. benign prostatic hyperplasia, or BPH) are major health problems among American men. The growth of the prostate gland occurs in various stages throughout the lifespan of male individuals, and both prostate cancer and BPH are the result of aberrant cellular turnover and growth. However, this process of cellular turnover is very poorly understood, particularly in humans. The goal of this project is to use AMS to measure Carbon-14 levels in the DNA of prostate cells as a result of Cold War atomic bomb testing. Measurement of Carbon-14 in the post-bomb testing era allows an accurate assessment of tissue turnover and cellular birth-dates; such an approach allows important questions in prostate biology research to be answered regarding the etiology of prostate disease. Methods: DNA will be extracted from tissue specimens, quality controlled, and submitted for Carbon-14 analyses using AMS. Tissue specimens will included archived tissue specimens from our institution (1955  present), as well as fresh prostate tissue specimens from patients currently at our institution. Carbon-14/Carbon-12 ratios will be compared to the known atmospheric Carbon-14 levels, as previously reported, and tissue and cellular "dates of birth" will be determined. Anticipated Results: We anticipate that AMS will accurately determine the rate of turnover within the prostate cellular compartments, and that such Carbon-14 levels will indicate the time of disease development when compared between normal prostate, prostate cancers, and BPH. Furthermore, such an approach will document the average lifespan of the various cell types within the prostate gland, such as the long-lived prostate stem cell. Finally, data generated from these studies will greatly facilitate the generation of new hypotheses regarding prostate function and disease etiology. Impact: Data generated from these studies will have an immediate impact on the field of prostate cancer research and will facilitate answers to essential - and as yet unanswered - questions pertaining to prostate growth, maintenance, and the etiology of prostate disease.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 简介：前列腺癌和前列腺肥大（即良性前列腺增生症，BPH）等前列腺疾病是美国男性的主要健康问题。 前列腺的生长发生在男性个体一生的各个阶段，前列腺癌和前列腺增生症都是细胞更新和生长异常的结果。 然而，人们对这种细胞更新过程知之甚少，尤其是在人类中。 该项目的目标是利用 AMS 测量冷战原子弹测试结果中前列腺细胞 DNA 中碳 14 的水平。 在后炸弹测试时代测量 Carbon-14 可以准确评估组织更新和细胞出生日期；这种方法可以回答前列腺生物学研究中有关前列腺疾病病因学的重要问题。 方法：从组织样本中提取 DNA，进行质量控制，并使用 AMS 进行 Carbon-14 分析。 组织标本将包括我们机构存档的组织标本（1955 年至今），以及来自我们机构目前患者的新鲜前列腺组织标本。 如先前报道的那样，碳 14/碳 12 比率将与已知的大气碳 14 水平进行比较，并确定组织和细胞的“出生日期”。 预期结果：我们预计 AMS 将准确确定前列腺细胞区室内的周转率，并且与正常前列腺、前列腺癌和 BPH 相比，此类 Carbon-14 水平将指示疾病发展的时间。 此外，这种方法将记录前列腺内各种细胞类型的平均寿命，例如长寿的前列腺干细胞。 最后，这些研究产生的数据将极大地促进有关前列腺功能和疾病病因学的新假设的产生。 影响：这些研究产生的数据将对前列腺癌研究领域产生直接影响，并将有助于回答有关前列腺生长、维持和前列腺疾病病因学的基本问题（但尚未得到解答）。 简介：前列腺癌和前列腺肥大（即良性前列腺增生症，BPH）等前列腺疾病是美国男性的主要健康问题。 前列腺的生长发生在男性个体一生的各个阶段，前列腺癌和前列腺增生症都是细胞更新和生长异常的结果。 然而，人们对这种细胞更新过程知之甚少，尤其是在人类中。 该项目的目标是利用 AMS 测量冷战原子弹测试结果中前列腺细胞 DNA 中碳 14 的水平。 在后炸弹测试时代测量 Carbon-14 可以准确评估组织更新和细胞出生日期；这种方法可以回答前列腺生物学研究中有关前列腺疾病病因学的重要问题。 方法：从组织样本中提取 DNA，进行质量控制，并使用 AMS 进行 Carbon-14 分析。 组织标本将包括我们机构存档的组织标本（1955 年至今），以及来自我们机构目前患者的新鲜前列腺组织标本。 如先前报道的那样，碳 14/碳 12 比率将与已知的大气碳 14 水平进行比较，并确定组织和细胞的“出生日期”。 预期结果：我们预计 AMS 将准确确定前列腺细胞区室内的周转率，并且与正常前列腺、前列腺癌和 BPH 相比，此类 Carbon-14 水平将指示疾病发展的时间。 此外，这种方法将记录前列腺内各种细胞类型的平均寿命，例如长寿的前列腺干细胞。 最后，这些研究产生的数据将极大地促进有关前列腺功能和疾病病因学的新假设的产生。 影响：这些研究产生的数据将对前列腺癌研究领域产生直接影响，并将有助于回答有关前列腺生长、维持和前列腺疾病病因学的基本问题（但尚未得到解答）。