INTEGRATED FLUORESCENCE DIFFUSE OPTICAL TOMOGRAPHY & MICRO-CT

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 使用荧光探针是体内分子成像的重要方法，特别是在研究小动物癌症模型时。尽管如此，由于缺乏断层成像能力，荧光成像主要局限于浅层组织的地形图。荧光漫射光学层析成像(F-DOT)是一种能够解决三维空间中荧光团浓度分布问题的新兴技术。然而，现有的大多数技术需要光纤将光源和探测器与动物耦合，这大大限制了它的应用和可实现的分辨率。我们正在实施一种非接触式的F-DOT系统，它使用光栅扫描脉冲激光和时间选通的具有旋转状态的增强型CCD(ICCD)相机来解析动物的三维荧光图像。我们通过使用扫描连续波半导体激光器的体模研究证明了我们的初步结果。在脉冲激光和时间门控采集；正则化图像重建技术；以及与我们的Micro-CT系统同时进行多模式成像方面，进一步的研究正在进行中。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Using fluorescent probes is an important methodology for in vivo molecular imaging, particularly to study small animal cancer models. Nonetheless the lack of tomographical imaging capability is limiting fluorescence imaging primarily to topographical mapping of superficial tissue. Fluorescence diffuse optical tomography (F-DOT) is an emerging technology capable of resolving the distribution of fluorophore concentration in 3-D. However, the majority of existing techniques require fiber-optics to couple the light sources and detectors with the animal, which significantly restricts its applications and achievable resolution. We are implementing a non-contact F-DOT system that uses raster-scanning pulsed laser and time-gated intensified CCD (ICCD) camera with a rotational state for the animal to resolve 3-D fluorescence images. We demonstrate our preliminary result from a phantom study using a scanning continuous-wave diode laser. Further study is under way in the directions of pulsed laser and time-gated acquisition; regularized image reconstruction techniques; and simultaneous multimodal imaging with our micro-CT system.