INTEGRATED FLUORESCENCE DIFFUSE OPTICAL TOMOGRAPHY & MICRO-CT

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 使用荧光探针是用于体内分子成像的重要方法，特别是用于研究小动物癌症模型。尽管如此，断层成像能力的缺乏限制了荧光成像主要用于浅表组织的地形图绘制。荧光扩散光学层析成像（F-DOT）是一种能够分辨荧光团浓度三维分布的新兴技术。然而，大多数现有技术需要光纤来将光源和检测器与动物耦合，这显著限制了其应用和可实现的分辨率。我们正在实施一种非接触式F-DOT系统，该系统使用光栅扫描脉冲激光和时间选通增强型CCD（ICCD）相机，具有旋转状态，用于动物解析3-D荧光图像。我们证明了我们的初步结果，从幻影研究使用扫描连续波二极管激光器。进一步的研究正在进行中的脉冲激光和时间选通采集的方向;正规化的图像重建技术;和同时多模态成像与我们的微型CT系统。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Using fluorescent probes is an important methodology for in vivo molecular imaging, particularly to study small animal cancer models. Nonetheless the lack of tomographical imaging capability is limiting fluorescence imaging primarily to topographical mapping of superficial tissue. Fluorescence diffuse optical tomography (F-DOT) is an emerging technology capable of resolving the distribution of fluorophore concentration in 3-D. However, the majority of existing techniques require fiber-optics to couple the light sources and detectors with the animal, which significantly restricts its applications and achievable resolution. We are implementing a non-contact F-DOT system that uses raster-scanning pulsed laser and time-gated intensified CCD (ICCD) camera with a rotational state for the animal to resolve 3-D fluorescence images. We demonstrate our preliminary result from a phantom study using a scanning continuous-wave diode laser. Further study is under way in the directions of pulsed laser and time-gated acquisition; regularized image reconstruction techniques; and simultaneous multimodal imaging with our micro-CT system.