TRANSGENIC INHIBITION OF MICRO-RNAS IN MOUSE BRAIN

小鼠脑中 MICRO-RNAS 的转基因抑制

• 批准号: 8171605
• 负责人: PETER JEFF NICHOLLS
• 金额: $ 0.55万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171605
• 关键词: Atrophic; Brain; Brain region; Computer Retrieval of Information on Scientific Projects Database; Corpus striatum structure; Foxes; Funding; Grant; Hippocampus (Brain); Huntington Disease; Hypothalamic structure; Institution; Microscopy; Modeling; Morus; Mouse Strains; Mus; Neocortex; Phenotype; Research; Research Personnel; Resolution; Resources; Source; Testing; Transgenic Organisms; United States National Institutes of Health; mouse model

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. High resolution MR microscopy will help assess possible morphological alterations in this new strain of mice. Our hypothesis is that these newly generated mouse is a model for Huntington's disease and we are going to use MR to test this hypothesis by assessing morphometric changes in regions known to be involved in Huntington's disease, most prominently the the striatum. Few exisitng studies have assessed morphometric changes in mouse model's of Huntington's disease and these studies include few large regions of the brain (Mori, 2010, Fox 2009). In addition, automated segmentation will offer information on whether other regions, including the hippocampus, hypothalamus, and neocortex are atrophied in this mouse model. We expect thus that the use of high resolution MRM will allow a comprehensive anatomical phenotyping of this new line of mice.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 高分辨率磁共振显微镜将有助于评估这种新品系小鼠可能的形态变化。 我们的假设是，这些新产生的小鼠是亨廷顿氏病的模型，我们将使用磁共振通过评估与亨廷顿氏病有关的已知区域的形态变化来检验这一假设，最突出的是纹状体。现有的研究很少评估亨廷顿病小鼠模型的形态计量学变化，这些研究包括很少的大脑大区域(Mori，2010，Fox 2009)。此外，自动分割将提供包括海马体、下丘脑和新皮质在内的其他区域在该小鼠模型中是否萎缩的信息。因此，我们期望高分辨率MRM的使用将允许对这一新品系的小鼠进行全面的解剖表型分析。