TRANSGENIC INHIBITION OF MICRO-RNAS IN MOUSE BRAIN

小鼠脑中 MICRO-RNAS 的转基因抑制

• 批准号: 8363178
• 负责人: PETER JEFF NICHOLLS
• 金额: $ 0.61万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363178
• 关键词: Atrophic; Brain; Brain region; Corpus striatum structure; Foxes; Funding; Grant; Hippocampus (Brain); Huntington Disease; Hypothalamic structure; Microscopy; Modeling; Morus; Mouse Strains; Mus; National Center for Research Resources; Neocortex; Phenotype; Principal Investigator; Research; Research Infrastructure; Resolution; Resources; Source; Testing; Transgenic Organisms; United States National Institutes of Health; cost; mouse model

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. High resolution MR microscopy will help assess possible morphological alterations in this new strain of mice. Our hypothesis is that these newly generated mouse is a model for Huntington's disease and we are going to use MR to test this hypothesis by assessing morphometric changes in regions known to be involved in Huntington's disease, most prominently the the striatum. Few exisitng studies have assessed morphometric changes in mouse model's of Huntington's disease and these studies include few large regions of the brain (Mori, 2010, Fox 2009). In addition, automated segmentation will offer information on whether other regions, including the hippocampus, hypothalamus, and neocortex are atrophied in this mouse model. We expect thus that the use of high resolution MRM will allow a comprehensive anatomical phenotyping of this new line of mice.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 高分辨率MR显微镜将有助于评估这种新品系小鼠可能的形态学改变。 我们的假设是，这些新产生的小鼠是亨廷顿氏病的模型，我们将使用MR通过评估已知与亨廷顿氏病有关的区域的形态学变化来验证这一假设，最突出的是纹状体。很少有研究评估了亨廷顿病小鼠模型的形态学变化，这些研究包括很少的大脑大区域（Mori，2010，Fox 2009）。此外，自动分割将提供有关其他区域（包括海马体、下丘脑和新皮层）是否在该小鼠模型中萎缩的信息。因此，我们期望使用高分辨率MRM将允许对这种新品系小鼠进行全面的解剖学表型分析。