APPLICATION OF PTMAP2 FOR PROTEIN POSTTRANSLATIONAL MODIFICATION ANALYSIS

PTMAP2 在蛋白质翻译后修饰分析中的应用

• 批准号: 8171932
• 负责人: YINGMING ZHAO
• 金额: $ 0.14万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2013-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8171932
• 关键词: Cell physiology; Computer Retrieval of Information on Scientific Projects Database; Data Files; Escherichia coli Proteins; Funding; Grant; Institution; Mass Spectrum Analysis; Organism; Play; Post-Translational Protein Processing; Protein Databases; Proteins; Research; Research Personnel; Resources; Role; Source; Testing; United States National Institutes of Health; genome-wide; protein function; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Posttranslational modifications (PTMs) play essential roles in protein function and cellular physiology. Unrestrictive identification of all types of PTMs using mass spectrometry is a powerful approach. Previous efforts have allowed us to successfully develop PTMap v2.0, a tool for genome-wide characterization of protein posttranslational modifications. Initial tests demonstrate that PTMap2 is capable to identify all types of PTMs in E. coli protein database from a single mass spectrometry data file. Here we propose to extend the application of PTMap2 tool to identify unknown PTMs in diverse cellular organisms.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 翻译后修饰（PTM）在蛋白质功能和细胞生理学中起着重要作用。使用质谱法对所有类型的PTM的不受限制识别是一种强大的方法。以前的努力使我们能够成功开发PTMAP V2.0，这是蛋白质翻译后修饰的全基因组表征的工具。初始测试表明，PTMAP2能够从单个质谱数据文件中识别大肠杆菌蛋白数据库中的所有类型的PTM。在这里，我们建议扩展PTMAP2工具的应用，以识别各种细胞生物中未知的PTM。