APPLICATION OF PTMAP2 FOR PROTEIN POSTTRANSLATIONAL MODIFICATION ANALYSIS

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 翻译后修饰在蛋白质功能和细胞生理中起着重要作用。使用质谱法对所有类型的PTM进行非限制性鉴定是一种强有力的方法。先前的努力使我们能够成功地开发PTMap v2.0，这是一种用于蛋白质翻译后修饰的全基因组表征的工具。初步测试表明，PTMap 2能够识别大肠杆菌中所有类型的PTM。coli蛋白质数据库中从单个质谱数据文件。在这里，我们建议扩展PTMap 2工具的应用，以确定未知的PTM在不同的细胞生物体。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Posttranslational modifications (PTMs) play essential roles in protein function and cellular physiology. Unrestrictive identification of all types of PTMs using mass spectrometry is a powerful approach. Previous efforts have allowed us to successfully develop PTMap v2.0, a tool for genome-wide characterization of protein posttranslational modifications. Initial tests demonstrate that PTMap2 is capable to identify all types of PTMs in E. coli protein database from a single mass spectrometry data file. Here we propose to extend the application of PTMap2 tool to identify unknown PTMs in diverse cellular organisms.