EFFECT OF PH ON MYOFILAMENT LATTICE SPACING/VOLUME

pH 对肌丝晶格间距/体积的影响

• 批准号: 8168644
• 负责人: Gerrie P Farman
• 金额: $ 1.8万
• 依托单位: ILLINOIS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2010-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8168644
• 关键词: Blood flow; Calcium; Cardiac; Cells; Computer Retrieval of Information on Scientific Projects Database; Filament; Funding; Goals; Grant; Heart; Institution; Ischemia; Microfilaments; Myocardial Infarction; Physiological; Proteins; Research; Research Personnel; Resources; Role; Skeletal Muscle; Source; System; United States National Institutes of Health; Work; base; interest

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The physiological impact of ischemia, the reduced blood flow in the heart, such as that observed during heart attacks has been of much interest to the cardiac field. Yet of all the physiological changes that occur the one least studied, or understood, is the impact of the inherent pH changes within the cells and how they might alter the native interactions between the filament proteins responsible for doing the work of the heart. Previous work in this field has examined the role of precise pH based changes in skeletal muscle and how that impacts the arrangement of these filament proteins, yet this work is nearly two decades old and never examined the impact of pH changes specifically in the cardiac system. Our goal was to examine the effects of altering the internal pH of intact paced cardiac trabeculae on the filament proteins and how that might correlate with the changes in force and calcium handling previously examined.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 缺血的生理影响，心脏中的血液流量降低，例如心脏病发作期间观察到的心脏田地引起了人们的关注。然而，在研究或理解的所有生理变化中，细胞内固有的pH变化的影响以及它们如何改变负责从事心脏工作的细丝蛋白之间的天然相互作用。该领域的先前工作已经检查了基于精确的骨骼肌肉变化的作用，以及如何影响这些细丝蛋白的排列，但是这项工作已近二十年，从未研究过pH变化在心脏系统中的影响。我们的目标是检查改变完整节奏心脏小梁对细丝蛋白的内部pH的影响，以及这可能与先前检查的力和钙处理的变化有关。