VIRAL LOCALIZATION BY IN SITU HYBRIDIZATION

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 猴免疫缺陷病毒(SIV)和SIV/HIV嵌合病毒(SHIV)感染亚洲猕猴是研究HIV感染和艾滋病发病机制的重要动物模型。此外，该模型还为评估旨在预防人类感染或艾滋病的疫苗和抗逆转录病毒药物的有效性提供了一个挑战系统。我们设计了几种分子探针，用于定位感染SIV和SIV不同分离株和克隆的猴子组织中的病毒RNA。这些探针被用来测量感染猴子组织中感染细胞的数量和分布。我们也有与其他几种病毒病原体的目标RNA或DNA序列杂交的探针，包括HIV、SRV-1、CMV、SV40、EBV、赛米里疱疹病毒和微小病毒。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The infection of Asian macaque monkeys with simian immunodeficiency virus (SIV) and SIV/HIV chimeric viruses (SHIVs) serves as an important animal model for studying the pathogenesis of HIV infection and AIDS. In addition, this model provides a challenge system for evaluating the efficacy of vaccines and antiretroviral agents designed to prevent infection or AIDS in humans. We have designed several molecular probes for localizing viral RNA in tissues of monkeys infected with various isolates and clones of SIV and SHIV. These probes are used to measure the quantity and distribution of infected cells in the tissues of infected monkeys. We also have probes that hybridize with target RNA or DNA sequences of several other viral pathogens, including HIV, SRV-1, CMV, SV40, EBV, Herpes saimiri, and parvovirus.