HISTOPATHOLOGY, IMMUNOHISTOCHEMISTRY, AND IN SITU HYBRIDIZATION SERVICES

组织病理学、免疫组织化学和原位杂交服务

• 批准号: 8357945
• 负责人: Susan V. Westmoreland
• 金额: $ 6.84万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8357945
• 关键词: Acquired Immunodeficiency Syndrome; Animal Model; Anti-Retroviral Agents; Antibodies; Asians; Cells; Comparative Pathology; Consultations; Cytomegalovirus; DNA Sequence; Diagnostic; Electron Microscopy; Experimental Pathology; Formalin; Freezing; Funding; Grant; HIV; HIV Infections; Hematoxylin and Eosin Staining Method; Histology; Histopathology; Housing; Human; Immunohistochemistry; In Situ Hybridization; Infection; Infection prevention; Infectious Agent; Institution; Macaca; Measures; Methods; Modeling; Molecular Probes; Monkeys; National Center for Research Resources; New England; Paraffin Embedding; Parvovirus; Pathogenesis; Pathology; Postdoctoral Fellow; Primates; Principal Investigator; Protocols documentation; RNA Sequences; Research; Research Infrastructure; Research Personnel; Resources; SIV; SRV-1; Saimiri; Services; Simian virus 40; Source; System; Testing; Tissues; United States National Institutes of Health; Viral; Virus; base; cost; design; graduate student; improved; investigator training; molecular pathology; nonhuman primate; pathogen; vaccine efficacy; viral RNA

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Pathology services were provided to a variety of investigators both at the center and from outside institutions. Services included routine hematoxylin and eosin histology, molecular pathology (immunohistochemistry and in situ hybridization), electron microscopy, and histopathology consultation. In order to improve our diagnostic pathology capabilities, we systematically evaluate commercially available antibodies directed against human cells/tissues and infectious agents for application in the nonhuman primate species housed at the NEPRC. Antibodies are selected for potential applications in diagnostic and/or experimental pathology, and for reactivity in either snap-frozen or formalin-fixed, paraffin-embedded tissues. In addition, the Division of Comparative Pathology receives multiple requests each year to test antibodies and develop IHC protocols for investigators in our Division, other NEPRC Divisions, and collaborating investigators. The infection of Asian macaque monkeys with simian immunodeficiency virus (SIV) and SIV/HIV chimeric viruses (SHIVs) serves as an important animal model for studying the pathogenesis of HIV infection and AIDS. In addition, this model provides a challenge system for evaluating the efficacy of vaccines and antiretroviral agents designed to prevent infection or AIDS in humans. We have designed several molecular probes for localizing viral RNA in tissues of monkeys infected with various isolates and clones of SIV and SHIV. These probes are used to measure the quantity and distribution of infected cells in the tissues of infected monkeys. We also have probes that hybridize with target RNA or DNA sequences of several other viral pathogens, including HIV, SRV-1, CMV, SV40, EBV, Herpes saimiri, and parvovirus. Pathology support is an essential core function that enhances colony management and research efforts by a number of institutions and investigators. The Division of Comparative Pathology also trains investigators, technicians, postdoctoral fellows and graduate students in immunohistochemistry methods. AIDS related and non-AIDS related.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 病理学服务提供给各种研究者在中心和外部机构。服务包括常规苏木精和伊红组织学、分子病理学（免疫组织化学和原位杂交）、电子显微镜和组织病理学咨询。为了提高我们的诊断病理学能力，我们系统地评估了针对人类细胞/组织和传染性病原体的市售抗体，以应用于NEPRC饲养的非人灵长类动物物种。 选择抗体用于诊断和/或实验病理学中的潜在应用，以及用于速冻或福尔马林固定的石蜡包埋组织中的反应性。 此外，比较病理学部门每年都会收到多个请求，要求为我们部门的研究人员，其他NEPRC部门和合作研究人员测试抗体并制定IHC方案。 猴免疫缺陷病毒（SIV）和SIV/HIV嵌合病毒（SHIVs）感染亚洲猕猴是研究HIV感染和AIDS发病机制的重要动物模型。 此外，该模型还提供了一个挑战系统，用于评估旨在预防人类感染或艾滋病的疫苗和抗逆转录病毒药物的功效。 我们设计了几种分子探针，用于定位感染SIV和SHIV的各种分离株和克隆的猴组织中的病毒RNA。 这些探针用于测量受感染猴子组织中受感染细胞的数量和分布。 我们也有探针与其他几种病毒病原体的靶RNA或DNA序列杂交，包括HIV，SRV-1，CMV，SV 40，EBV，猴疱疹病毒和细小病毒。 病理学支持是一个必不可少的核心功能，它增强了许多机构和研究人员的菌落管理和研究工作。比较病理学系还培训研究人员、技术人员、博士后研究员和研究生免疫组织化学方法。 与艾滋病有关和无关。