ESTABLISHMENT OF SPF RHESUS MACAQUE BREEDING COLONY: HIV IMMUNOLOGY

SPF级恒河猴繁育群体的建立：HIV免疫学

• 批准号: 8173549
• 负责人: KEITH G. MANSFIELD
• 金额: $ 13.57万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173549
• 关键词: Acquired Immunodeficiency Syndrome; Animal Model; Animals; Biological; Breeding; Cercopithecine Herpesvirus 1; Computer Retrieval of Information on Scientific Projects Database; Confounding Factors (Epidemiology); Disease; Disease Progression; Funding; Genetic screening method; Goals; Grant; Health; Housing; Human; Immunity; Immunology; Infection; Infectious Agent; Institution; Lead; Macaca; Macaca mulatta; Mission; Pathogenesis; Research; Research Personnel; Resources; Role; SIV; Scientist; Simian Retroviruses; Simian T-lymphotropic virus 1; Source; Specific Pathogen Frees; Testing; United States National Institutes of Health; Universities; Viral; Virus; base; germ free condition; meetings; programs

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The SIV-infected rhesus macaque has emerged as the premiere animal model of human AIDS and has lead to important advances in understanding aspects of disease pathogenesis, the role of viral determinants on disease progression and the impact of host immunity in controlling viral replication. The limited availability of Indian-origin rhesus macaques (Macaca mulatta) specific pathogen free of B virus (BV), Simian T lymphotropic Virus (STLV-1), Simian Retrovirus Type D (SRV-D) and Simian Immunodeficiency Virus (SIV) has become an impediment to investigators conducting animal model based AIDS-related research. Such animals are important for reasons of colony health, and biosafety and to reduce confounding variables associated with co-infection with these viruses. In addition to these requirements, animals free of other infectious agents or of defined MHC type have become increasingly important in the implementation of certain research programs. The NEPRC first established a breeding colony free of BV, STLV-1, SRV-D and SIV in 1988 and this colony has been instrumental in meeting its national resource mission by providing well-defined animals to core and collaborating scientists for use in AIDS-related research. In recognition of the importance of this colony, Harvard University recently funded the construction of a $3.1 M specific pathogen free rhesus macaque breeding facility and a $7.1M SPF macaque housing and testing facility. This application proposes to expand and further refine the existing NEPRC colony to take advantage of these and other improvements. To accomplish this goal we propose the following specific aims: 1) To expand the NEPRC breeding colony specific pathogen free of BV, STLV-1, SRV-D and SIV through internal and external recruitment of well-defined Indian-origin rhesus macaques; 2) To continue virologic testing of the SPF colony and expand microbiologic assessment of the SPF and "super-clean" breeding groups; and 3) To continue MHC typing and genetic testing with the goal of enhancing colony management and the biological resource.

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 描述(申请人提供)：SIV感染的恒河猴已成为人类艾滋病的主要动物模型，并在理解疾病发病机制、病毒决定因素在疾病进展中的作用以及宿主免疫在控制病毒复制方面的影响方面取得了重要进展。印度恒河猴(Macaca Mulatta)特异性病原体不含B病毒(BV)、猿猴T淋巴细胞白血病病毒(STLV-1)、猿猴D型逆转录病毒(SRV-D)和猿猴免疫缺陷病毒(SIV)的有限供应，已成为阻碍研究人员进行基于动物模型的艾滋病相关研究的障碍。这类动物对于群体健康和生物安全以及减少与这些病毒混合感染相关的混淆变量来说是重要的。除了这些要求外，没有其他感染源或确定的MHC类型的动物在某些研究计划的实施中变得越来越重要。NEPRC于1988年首次建立了一个没有BV、STLV-1、SRV-D和SIV的繁育群体，该群体为履行其国家资源使命发挥了重要作用，为核心科学家和合作科学家提供了明确的动物，用于与艾滋病有关的研究。认识到这个群体的重要性，哈佛大学最近出资310万美元建造了一个无特定病原体的恒河猴繁育设施，以及一个价值710万美元的SPF猕猴住房和测试设施。这项申请建议扩大和进一步完善现有的非国家核可再生能源机构群体，以利用这些改进和其他改进。 为实现这一目标，我们提出了以下具体目标： 1)通过内部和外部招募定义明确的印度原种猕猴，扩大NEPRC养殖群体中不含BV、STLV-1、SRV-D和SIV的特定病原体；2)继续对SPF群体进行病毒学测试，并扩大对SPF和“超清洁”繁殖群体的微生物学评估；以及3)继续MHC分型和基因测试，以加强群体管理和生物资源。