ESTABLISHMENT OF SPF RHESUS MACAQUE BREEDING COLONY: HIV VACCINE DVMT

SPF 恒河猴繁育群体的建立：HIV 疫苗 DVMT

• 批准号: 8173552
• 负责人: KEITH G. MANSFIELD
• 金额: $ 13.57万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8173552
• 关键词: Acquired Immunodeficiency Syndrome; Animal Model; Animals; Biological; Breeding; Cercopithecine Herpesvirus 1; Computer Retrieval of Information on Scientific Projects Database; Confounding Factors (Epidemiology); Disease; Disease Progression; Funding; Genetic screening method; Goals; Grant; Health; Housing; Human; Immunity; Infection; Infectious Agent; Institution; Lead; Macaca; Macaca mulatta; Mission; Pathogenesis; Research; Research Personnel; Resources; Role; SIV; Scientist; Simian Retroviruses; Simian T-lymphotropic virus 1; Source; Specific Pathogen Frees; Testing; United States National Institutes of Health; Universities; Vaccines; Viral; Virus; base; germ free condition; meetings; programs

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The SIV-infected rhesus macaque has emerged as the premiere animal model of human AIDS and has lead to important advances in understanding aspects of disease pathogenesis, the role of viral determinants on disease progression and the impact of host immunity in controlling viral replication. The limited availability of Indian-origin rhesus macaques (Macaca mulatta) specific pathogen free of B virus (BV), Simian T lymphotropic Virus (STLV-1), Simian Retrovirus Type D (SRV-D) and Simian Immunodeficiency Virus (SIV) has become an impediment to investigators conducting animal model based AIDS-related research. Such animals are important for reasons of colony health, and biosafety and to reduce confounding variables associated with co-infection with these viruses. In addition to these requirements, animals free of other infectious agents or of defined MHC type have become increasingly important in the implementation of certain research programs. The NEPRC first established a breeding colony free of BV, STLV-1, SRV-D and SIV in 1988 and this colony has been instrumental in meeting its national resource mission by providing well-defined animals to core and collaborating scientists for use in AIDS-related research. In recognition of the importance of this colony, Harvard University recently funded the construction of a $3.1 M specific pathogen free rhesus macaque breeding facility and a $7.1M SPF macaque housing and testing facility. This application proposes to expand and further refine the existing NEPRC colony to take advantage of these and other improvements. To accomplish this goal we propose the following specific aims: 1) To expand the NEPRC breeding colony specific pathogen free of BV, STLV-1, SRV-D and SIV through internal and external recruitment of well-defined Indian-origin rhesus macaques; 2) To continue virologic testing of the SPF colony and expand microbiologic assessment of the SPF and "super-clean" breeding groups; and 3) To continue MHC typing and genetic testing with the goal of enhancing colony management and the biological resource.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目和 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 描述（由申请人提供）：感染 SIV 的恒河猴已成为人类艾滋病的首要动物模型，并在理解疾病发病机制、病毒决定因素对疾病进展的作用以及宿主免疫对控制病毒复制的影响方面取得了重要进展。印度原产恒河猴（Macaca mulatta）不含 B 病毒（BV）、猿猴 T 淋巴细胞病毒（STLV-1）、猿猴逆转录病毒 D 型（SRV-D）和猿猴免疫缺陷病毒（SIV）的特定病原体的供应有限，这已成为研究人员进行基于动物模型的艾滋病相关研究的障碍。这些动物对于群体健康和生物安全以及减少与这些病毒共同感染相关的混杂变量非常重要。除了这些要求外，不含其他传染源或具有明确 MHC 类型的动物在某些研究计划的实施中变得越来越重要。 NEPRC 于 1988 年首次建立了一个不含 BV、STLV-1、SRV-D 和 SIV 的繁殖群，该群通过向核心科学家和合作科学家提供用于艾滋病相关研究的明确动物，在实现其国家资源使命方面发挥了重要作用。哈佛大学认识到该群体的重要性，最近资助建造了价值 310 万美元的无特定病原体恒河猴繁殖设施和价值 710 万美元的 SPF 猕猴饲养和测试设施。该申请建议扩大和进一步完善现有的 NEPRC 集群，以利用这些和其他改进。 为了实现这一目标，我们提出以下具体目标： 1) 通过内部和外部招募明确的印度产恒河猴，扩大 NEPRC 繁殖群体，使其不含 BV、STLV-1、SRV-D 和 SIV 特定病原体； 2) 继续对SPF群体进行病毒学检测，并扩大对SPF和“超洁净”育种群体的微生物学评估； 3) 继续进行MHC分型和基因检测，以加强菌落管理和生物资源。