ESTABLISHMENT OF SPF RHESUS MACAQUE BREEDING COLONY: HIV GENOME

该子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心赠款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 表示子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 描述（由申请人提供）：SIV感染的恒河猴已成为人类艾滋病的首要动物模型，并在理解疾病发病机制、病毒决定因素对疾病进展的作用以及宿主免疫力对控制病毒复制的影响方面取得了重要进展。不含B病毒（BV）、猿猴T淋巴细胞病毒（STLV-1）、猿猴D型逆转录病毒（SRV-D）和猿猴免疫缺陷病毒（SIV）的印度原产恒河猴（Macaca mulatta）特异性病原体的有限可用性已成为研究人员进行基于动物模型的艾滋病相关研究的障碍。出于群体健康和生物安全的原因，以及为了减少与这些病毒的共感染相关的混杂变量，这些动物是重要的。除了这些要求之外，在某些研究项目的实施中，没有其他传染性病原体或确定的MHC类型的动物变得越来越重要。NEPRC于1988年首次建立了一个无BV、STLV-1、SRV-D和SIV的繁殖群，该繁殖群通过向核心和合作科学家提供定义明确的动物用于艾滋病相关研究，在满足其国家资源使命方面发挥了重要作用。认识到这个群体的重要性，哈佛大学最近资助建造了一个310万美元的无特定病原体恒河猴繁殖设施和一个710万美元的SPF猕猴饲养和测试设施。本申请建议扩展和进一步完善现有的NEPRC集群，以利用这些和其他改进。 为实现这一目标，我们提出以下具体目标： 1)通过内部和外部招募明确的印度恒河猴，扩大无BV、STLV-1、SRV-D和SIV的NEPRC繁殖群体特异性病原体; 2）继续SPF群体的病毒学检测，扩大SPF和“超净”繁殖群体的微生物学评估;（3）继续进行MHC分型和基因检测，以加强群体管理和生物资源。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. DESCRIPTION (provided by applicant): The SIV-infected rhesus macaque has emerged as the premiere animal model of human AIDS and has lead to important advances in understanding aspects of disease pathogenesis, the role of viral determinants on disease progression and the impact of host immunity in controlling viral replication. The limited availability of Indian-origin rhesus macaques (Macaca mulatta) specific pathogen free of B virus (BV), Simian T lymphotropic Virus (STLV-1), Simian Retrovirus Type D (SRV-D) and Simian Immunodeficiency Virus (SIV) has become an impediment to investigators conducting animal model based AIDS-related research. Such animals are important for reasons of colony health, and biosafety and to reduce confounding variables associated with co-infection with these viruses. In addition to these requirements, animals free of other infectious agents or of defined MHC type have become increasingly important in the implementation of certain research programs. The NEPRC first established a breeding colony free of BV, STLV-1, SRV-D and SIV in 1988 and this colony has been instrumental in meeting its national resource mission by providing well-defined animals to core and collaborating scientists for use in AIDS-related research. In recognition of the importance of this colony, Harvard University recently funded the construction of a $3.1 M specific pathogen free rhesus macaque breeding facility and a $7.1M SPF macaque housing and testing facility. This application proposes to expand and further refine the existing NEPRC colony to take advantage of these and other improvements. To accomplish this goal we propose the following specific aims: 1) To expand the NEPRC breeding colony specific pathogen free of BV, STLV-1, SRV-D and SIV through internal and external recruitment of well-defined Indian-origin rhesus macaques; 2) To continue virologic testing of the SPF colony and expand microbiologic assessment of the SPF and "super-clean" breeding groups; and 3) To continue MHC typing and genetic testing with the goal of enhancing colony management and the biological resource.