Lung Biological Specimens Core

肺生物标本核心

• 批准号: 8196617
• 负责人: Anne I. Sperling
• 金额: $ 22.81万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-25 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8196617
• 关键词: Affect; Asthma; Biocompatible Materials; Biological; Biological Assay; Biopsy; Blood; Blood Cells; Blood specimen; Bronchoalveolar Lavage; Bronchoalveolar Lavage Fluid; Bronchoscopy; CD4 Positive T Lymphocytes; Cell Separation; Cells; Clinical; Clinical Trials Data Monitoring Committees; Collecting Cell; Collection; Core Facility; DNA; Databases; Doctor of Philosophy; Ensure; Epithelial Cells; Familiarity; Flow Cytometry; Gene Expression; Genes; Genetic; Genotype; HLA G antigen; Immune; Immunohistochemistry; Infiltration; Label; Liquid substance; Lung; Lymphocyte; Lymphocyte Subset; Medicine; MicroRNAs; Mononuclear; Morbidity - disease rate; National Institute of Allergy and Infectious Disease; Pathogenesis; Patient Recruitments; Performance; Peripheral Blood Mononuclear Cell; Plasma; Process; Quality Control; RNA; RNA analysis; Regulation; Reporting; Research; Research Subjects; Role; Sampling; Smooth Muscle Myocytes; Specimen; Suppressor-Effector T-Lymphocytes; T-Lymphocyte; United States; Universities; Work; airway epithelium; airway inflammation; chemokine; cytokine; data management; eosinophil; established cell line; experience; laser capture microdissection; meetings; professor; programs; receptor; research study; respiratory smooth muscle; synthetic polymer Bioplex; tissue processing

The Lung Biospecimens Core will provide our AADCRC Program with processing of all blood and lung specimens to be collected for each project. This includes all tissue processing, isolation of cells from blood and bronchoalveolar lavage fluid. Isolation of DNA, RNA and miRNA, and performance of bioplex cytokine and chemokine assays. The Core is led by Anne Sperling, PhD, Associate Professor of Medicine, who has extensive experience in the administration of university-wide core facilities and deep familiarity with all the assays and isolations to be performed by this Core. A senior research technologist performs all assays and isolations and maintains rigorous quality control and record-keeping. Timely isolation and storage of biological materials is of the utmost importance in the successful completion of each of the projects in this program. This dedicated Core will meet these obligations.

肺生物标本中心将为我们的AADCRC项目提供所有血液和肺的处理