Lung Biological Specimens Core
Lung Biological Specimens Core
批准号:
8196617
负责人:
Anne I. Sperling
金额:
$22.81万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2011
资助国家:
美国
项目状态:
已结题
起止时间:
2011-07-25 至 2016-06-30
关键词:
AffectAsthmaBiocompatible MaterialsBiologicalBiological AssayBiopsyBloodBlood CellsBlood specimenBronchoalveolar LavageBronchoalveolar Lavage FluidBronchoscopyCD4 Positive T LymphocytesCell SeparationCellsClinicalClinical Trials Data Monitoring CommitteesCollecting CellCollectionCore FacilityDNADatabasesDoctor of PhilosophyEnsureEpithelial CellsFamiliarityFlow CytometryGene ExpressionGenesGeneticGenotypeHLA G antigenImmuneImmunohistochemistryInfiltrationLabelLiquid substanceLungLymphocyteLymphocyte SubsetMedicineMicroRNAsMononuclearMorbidity - disease rateNational Institute of Allergy and Infectious DiseasePathogenesisPatient RecruitmentsPerformancePeripheral Blood Mononuclear CellPlasmaProcessQuality ControlRNARNA analysisRegulationReportingResearchResearch SubjectsRoleSamplingSmooth Muscle MyocytesSpecimenSuppressor-Effector T-LymphocytesT-LymphocyteUnited StatesUniversitiesWorkairway epitheliumairway inflammationchemokinecytokinedata managementeosinophilestablished cell lineexperiencelaser capture microdissectionmeetingsprofessorprogramsreceptorresearch studyrespiratory smooth musclesynthetic polymer Bioplextissue processing
中文摘要
肺部生物样本核心将为我们的AADCRC计划提供所有血液和肺部样本的处理。
为每个项目收集样本。这包括所有的组织处理,从血液中分离细胞
和支气管肺泡灌洗液。DNA、RNA和miRNA的分离,以及bioplex细胞因子和
趋化因子测定。
核心是由安妮斯珀林,博士,医学副教授,谁拥有丰富的经验,
管理大学范围内的核心设施,并深入了解所有的测定和分离,
由这个核心来执行。高级研究技术人员执行所有测定和分离,并维护
严格的质量控制和记录保存。
及时隔离和储存生物材料对成功完成
这个项目中的每个项目。这一专门的核心将履行这些义务。
英文摘要
The Lung Biospecimens Core will provide our AADCRC Program with processing of all blood and lung
specimens to be collected for each project. This includes all tissue processing, isolation of cells from blood
and bronchoalveolar lavage fluid. Isolation of DNA, RNA and miRNA, and performance of bioplex cytokine and
chemokine assays.
The Core is led by Anne Sperling, PhD, Associate Professor of Medicine, who has extensive experience in
the administration of university-wide core facilities and deep familiarity with all the assays and isolations to
be performed by this Core. A senior research technologist performs all assays and isolations and maintains
rigorous quality control and record-keeping.
Timely isolation and storage of biological materials is of the utmost importance in the successful completion of
each of the projects in this program. This dedicated Core will meet these obligations.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Function of asthma- and allergic disease-associated risk variants and genes in lung immune cells
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批准号:10261991
-
项目类别:
-
资助金额:$47.46万
-
财政年份:2021
-
负责人:Anne I. Sperling
-
依托单位:
Function of asthma- and allergic disease-associated risk variants and genes in lung immunecells
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批准号:10827535
-
项目类别:
-
资助金额:$48.04万
-
财政年份:2021
-
负责人:Anne I. Sperling
-
依托单位:
Function of asthma- and allergic disease-associated risk variants and genes in lung immune cells
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批准号:10453777
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项目类别:
-
资助金额:$38.86万
-
财政年份:2021
-
负责人:Anne I. Sperling
-
依托单位:
IRF4+ respiratory dendritic cells in type 2 inflammatory responses
-
批准号:9311817
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项目类别:
-
资助金额:$39.72万
-
财政年份:2017
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负责人:Anne I. Sperling
-
依托单位:
Human Lung T cell subsets in Disease
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批准号:9169074
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项目类别:
-
资助金额:$23.7万
-
财政年份:2016
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负责人:Anne I. Sperling
-
依托单位:
HLA-G effector mechanisms in asthma
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批准号:8691382
-
项目类别:
-
资助金额:$6.67万
-
财政年份:2013
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负责人:Anne I. Sperling
-
依托单位:
Lung Biological Specimens Core
-
批准号:8380132
-
项目类别:
-
资助金额:$17.38万
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财政年份:2012
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负责人:Anne I. Sperling
-
依托单位:
HLA-G effector mechanisms in asthma
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批准号:8380125
-
项目类别:
-
资助金额:$40.18万
-
财政年份:2012
-
负责人:Anne I. Sperling
-
依托单位:
HLA-G effector mechanisms in asthma
-
批准号:8196611
-
项目类别:
-
资助金额:$32.14万
-
财政年份:2011
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负责人:Anne I. Sperling
-
依托单位:
Dendritic cell produced IL-33 in Th2 responses
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批准号:8104938
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项目类别:
-
资助金额:$22.86万
-
财政年份:2011
-
负责人:Anne I. Sperling
-
依托单位:
Dendritic cell produced IL-33 in Th2 responses
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批准号:8318053
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项目类别:
-
资助金额:$18.96万
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财政年份:2011
-
负责人:Anne I. Sperling
-
依托单位:
Mechanisms of Resolution in Experimental Asthma
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批准号:7879705
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项目类别:
-
资助金额:$1.11万
-
财政年份:2009
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负责人:Anne I. Sperling
-
依托单位:
Amnis ImageStream Analyzer
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批准号:7794015
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项目类别:
-
资助金额:$42.0万
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财政年份:2009
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负责人:Anne I. Sperling
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依托单位:
CD43 Regulation of Immune Responses
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批准号:7922800
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项目类别:
-
资助金额:$31.63万
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财政年份:2009
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负责人:Anne I. Sperling
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依托单位:
BD LSR II Flow Cytometer
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批准号:7595564
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项目类别:
-
资助金额:$22.38万
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财政年份:2009
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负责人:Anne I. Sperling
-
依托单位:
Immunology Core
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批准号:7700362
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项目类别:
-
资助金额:$19.65万
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财政年份:2008
-
负责人:Anne I. Sperling
-
依托单位:
FLOW CYTOMETRY
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批准号:7714277
-
项目类别:
-
资助金额:$10.4万
-
财政年份:2008
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负责人:Anne I. Sperling
-
依托单位:
FITCH MONOCLONAL ANTIBODY
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批准号:7714274
-
项目类别:
-
资助金额:$4.98万
-
财政年份:2008
-
负责人:Anne I. Sperling
-
依托单位:
Mechanisms of Resolution in Experimental Asthma
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批准号:8037131
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项目类别:
-
资助金额:$36.9万
-
财政年份:2007
-
负责人:Anne I. Sperling
-
依托单位:
Mechanisms of Resolution in Experimental Asthma
-
批准号:7267300
-
项目类别:
-
资助金额:$38.38万
-
财政年份:2007
-
负责人:Anne I. Sperling
-
依托单位:
海外基金