BD LSR II Flow Cytometer
BD LSR II 流式细胞仪
基本信息
- 批准号:7595564
- 负责人:
- 金额:$ 22.38万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2009
- 资助国家:美国
- 起止时间:2009-01-15 至 2010-01-14
- 项目状态:已结题
- 来源:
- 关键词:AntigensAreaArtsAsthmaAutoimmune DiseasesBiologicalBiomedical ResearchCancer Immunology ScienceCellsChicagoCore FacilityDetectionDiseaseEventFacultyFlow CytometryFluorescenceFundingHematopoiesisHost DefenseImmuneIndividualKnowledgeLasersLifeMalignant NeoplasmsMediatingMedical ResearchOperative Surgical ProceduresOrganResearchResearch PersonnelScienceStem Cell ResearchTechnologyTissuesTransplantationUnited States National Institutes of HealthUniversitiesbasecell typeexperienceinstrumentinstrumentationlung injurypublic health relevancetool
项目摘要
DESCRIPTION (provided by applicant): Multiparametric flow cytometry has proven to be an indispensable tool for numerous areas of biological and medical research at the University of Chicago. The ability to identify and enumerate individual living cells in a precise and rapid fashion has allowed for the characterization of rare cell types from any number of tissues and organs. The ability to detect such events is particularly crucial for the study of cancer and immune-mediated diseases. Previously, multiparametric flow cytometers have been able to identify many such cell types; however the limits of fluorescence detection have been reached on many commercial instruments. The necessity to resolve cell types with very low antigen expression has become evident in the area of stem cell research and hematopoiesis. Therefore, it is evident that instrumentation that will be able to detect multiple parameters simultaneously at high through-put rates, and also resolve these dimly fluorescent cell subtypes, is necessary for the continued advancement of the research at the University of Chicago. A new, high sensitivity, 4-laser, 14-parameter BD LSRII will allow for these detection criterion, and also alleviate some of the capacity issues currently experienced by the user base. A 15% increase in usage of the current facility's LSRII and the loss of the Cyan-ADP has forced the facility to begin leasing the requested LSRII. As such this proposal has been revised to request funding to buyout the leased instrument so that the major investigators may continue to perform their studies. Twelve major users with as many NIH funded projects focusing on a wide variety of biomedical science including hematopoiesis, asthma, host defense, lung injury, transplantation, autoimmune diseases as well as basic cancer and immunology research, will depend on this instrumentation. The efficiency of the usage of this instrument will be aided by its placement in the University of Chicago Flow Cytometry Core Facility on campus. By placing this instrument in the already established core facility on campus (serving >80 PI's), the faculty will be able to exploit the expert knowledge of the facility directors and staff in its operation and usage. Further, in the core facility equal access is provided to all University Investigators. Thus, this instrument will greatly benefit the University of Chicago by easing current capacity issues and providing state-of-the-art technology, allowing our investigators to remain at the forefront of their research. PUBLIC HEALTH RELEVANCE: Twelve NIH funded projects focusing on biomedical research areas such as hematopoiesis, asthma, host defense, lung injury, transplantation, autoimmune diseases, mechanisms of cancer and immunology will rely heavily on the requested instrumentation. State-of-the-art technologies employed on the requested instrument will help unveil the information necessary to answer these important questions.
描述(由申请人提供):多参数流式细胞术已被证明是芝加哥大学众多生物和医学研究领域不可或缺的工具。以精确和快速的方式识别和计数单个活细胞的能力允许表征来自任何数量的组织和器官的稀有细胞类型。检测这些事件的能力对于癌症和免疫介导的疾病的研究特别重要。以前,多参数流式细胞仪已经能够识别许多这样的细胞类型;然而,在许多商业仪器上已经达到荧光检测的极限。在干细胞研究和造血领域,解决抗原表达非常低的细胞类型的必要性已经变得显而易见。因此,很明显,仪器,将能够检测多个参数,同时在高通量率,并解决这些暗淡的荧光细胞亚型,是必要的研究在芝加哥大学的持续进步。新的高灵敏度、4激光、14参数BD LSRII将满足这些检测标准,并缓解用户群目前遇到的一些容量问题。当前设施的LSRII使用量增加15%以及Cyan-ADP的损失迫使该设施开始租赁所请求的LSRII。因此,对这一提案进行了修订,要求提供资金买断租赁的仪器,以便主要研究人员可以继续进行研究。12个主要用户与许多NIH资助的项目集中在各种各样的生物医学科学,包括造血,哮喘,宿主防御,肺损伤,移植,自身免疫性疾病以及基础癌症和免疫学研究,将依赖于这种仪器。将该仪器放置在校园内的芝加哥大学流式细胞术核心设施中,将有助于提高该仪器的使用效率。通过将该仪器放置在校园内已经建立的核心设施中(为80多个PI提供服务),教师将能够利用设施主管和工作人员在其操作和使用方面的专业知识。此外,在核心设施中,所有大学调查员都可以平等进入。因此,该仪器将大大有利于芝加哥大学缓解当前的能力问题,并提供最先进的技术,使我们的研究人员保持在他们的研究的最前沿。公共卫生关系:12个NIH资助的项目,重点是生物医学研究领域,如造血,哮喘,宿主防御,肺损伤,移植,自身免疫性疾病,癌症和免疫学的机制将在很大程度上依赖于所要求的仪器。所要求的仪器采用的最新技术将有助于揭示回答这些重要问题所需的信息。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Anne I. Sperling其他文献
Exopolysaccharide-treated Dendritic Cells Effectively Ameliorate Acute Graft vs Host Disease.
胞外多糖处理的树突状细胞可有效改善急性移植物抗宿主病。
- DOI:
10.1016/j.jtct.2023.10.023 - 发表时间:
2023 - 期刊:
- 影响因子:3.2
- 作者:
O. Kalinina;L. Minter;Anne I. Sperling;M. K. Hollinger;Phong Le;Barbara A. Osborne;Shubin Zhang;Patrick Stiff;Katherine L. Knight - 通讯作者:
Katherine L. Knight
Lung cDC1 and cDC2 dendritic cells priming naive CD8sup+/sup T cells emin situ/em prior to migration to draining lymph nodes
肺 cDC1 和 cDC2 树突状细胞在迁移至引流淋巴结之前原位初始 CD8+T 细胞的启动
- DOI:
10.1016/j.celrep.2023.113299 - 发表时间:
2023-10-31 - 期刊:
- 影响因子:6.900
- 作者:
Youhui Si;Yihan Wang;Qiaomu Tian;Qiang Wang;Jared M. Pollard;Pramod K. Srivastava;Aaron P. Esser-Kahn;Joel H. Collier;Anne I. Sperling;Anita S. Chong - 通讯作者:
Anita S. Chong
Integration of functional genomics and statistical fine-mapping systematically characterizes adult-onset and childhood-onset asthma genetic associations
- DOI:
10.1186/s13073-025-01459-z - 发表时间:
2025-04-10 - 期刊:
- 影响因子:11.200
- 作者:
Xiaoyuan Zhong;Robert Mitchell;Christine Billstrand;Emma E. Thompson;Noboru J. Sakabe;Ivy Aneas;Isabella M. Salamone;Jing Gu;Anne I. Sperling;Nathan Schoettler;Marcelo A. Nóbrega;Xin He;Carole Ober - 通讯作者:
Carole Ober
Anne I. Sperling的其他文献
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{{ truncateString('Anne I. Sperling', 18)}}的其他基金
Function of asthma- and allergic disease-associated risk variants and genes in lung immune cells
肺免疫细胞中哮喘和过敏性疾病相关风险变异和基因的功能
- 批准号:
10261991 - 财政年份:2021
- 资助金额:
$ 22.38万 - 项目类别:
Function of asthma- and allergic disease-associated risk variants and genes in lung immunecells
肺免疫细胞中哮喘和过敏性疾病相关风险变异和基因的功能
- 批准号:
10827535 - 财政年份:2021
- 资助金额:
$ 22.38万 - 项目类别:
Function of asthma- and allergic disease-associated risk variants and genes in lung immune cells
肺免疫细胞中哮喘和过敏性疾病相关风险变异和基因的功能
- 批准号:
10453777 - 财政年份:2021
- 资助金额:
$ 22.38万 - 项目类别:
IRF4+ respiratory dendritic cells in type 2 inflammatory responses
IRF4呼吸树突状细胞在2型炎症反应中的作用
- 批准号:
9311817 - 财政年份:2017
- 资助金额:
$ 22.38万 - 项目类别:
Dendritic cell produced IL-33 in Th2 responses
树突状细胞在 Th2 反应中产生 IL-33
- 批准号:
8104938 - 财政年份:2011
- 资助金额:
$ 22.38万 - 项目类别:
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