Immunology Core

为中西部地区RCE建议的免疫学核心设施(ICF)目前为教师提供服务 在芝加哥大学通过提供最先进的免疫技术和 测量分子、细胞和组织功能的定量分析方法。为 联盟成员，新的基于免疫的疗法的开发，如疫苗和新的 细胞因子，需要仔细监测科学终点，以确定生物学上的最佳 这些药物的有效剂量和时间表。ICF代表着一家拥有专业知识的成熟机构 为了监测和评估的目的处理人类和小鼠的细胞和组织 免疫反应。这种多参数免疫学分析将为研究人员提供 用于确定临床前和临床治疗的疗效的最新分析方法 有针对性的感染源。ICF目前提供各种服务和专业知识，以 它的用户。对于基础科学研究，该设施被许多不同领域的研究人员使用 用于研究细胞结构/功能以及临床转译研究的研究 应用和分子医学。该机构专门从事细胞免疫组织化学的研究。 在周围组织的上下文中进行检测，以及产生和生产新的 检测细胞和亚细胞成分所必需的单抗。ICF 还拥有专门的流式细胞仪小组，用于单细胞的分析检测和分选 使用多参数荧光技术的悬浮液和亚细胞组件。为 在人类患者的临床试验中，ICF进行检测以监测免疫反应。这 该服务使临床研究人员能够在参与的研究中测量免疫终点 研究对象。终点分析主要集中在人类T细胞反应上，但包括 其他免疫学读数的范围。因此，ICF可以为财团成员提供 用于开展研究和开发的专家免疫监测能力 项目。

The Immunology Core Facility (ICF) proposed for the Midwestern RCE currently serves the faculty at the University of Chicago by providing access to state-of-the-art immunological technologies and quantitative analytical approaches to measure molecular, cellular and tissue function. For consortium members, development of new immune-based therapies, such as vaccines and novel cytokines, requires careful monitoring of scientific endpoints to determine the optimal biologically active dose and schedule of these agents. The ICF represents an established facility with expertise in handling human and mouse cells and tissues for the purpose of monitoring and evaluating immunological responses. This multi-parameter immunologic analysis will provide researchers with state-of-the-art assays for determining the efficacy of pre-clinical and clinical therapies against targeted infectious agents. The ICF currently provides a wide variety of services and expertise to its users. For basic science research, the facility is utilized by researchers in many different areas of study for investigation of cellular structure/function, as well as for translational research in clinical applications and molecular medicine. The facility specializes in immunohistochemistry for cellular detection within the context of the surrounding tissue, and generation and production of novel monoclonal antibodies necessary for the detection of cells and subcellular components. The ICF also has an expert flow cytometry group for the analytical detection and sorting of single cell suspensions and subcellular components using multi-parameter fluorescence technology. For clinical trials in human patients, the ICF performs assays to monitor immune responses. This service enables clinical researchers to measure immunologic endpoints in participating study subjects. The endpoint assays are primarily focused on human T cell responses, but include a range of other immunological readouts. Thus, the ICF can provide consortium members with expert immunologic monitoring capabilities with which to carry out research and developmental projects.