Project 2: Exposure Assessment of Macondo Oil Spill in Affected Communities

项目 2：受影响社区马孔多漏油事件暴露评估

• 批准号: 8238912
• 负责人: G.A. Shakeel Ansari
• 金额: $ 18.79万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-27 至 2016-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8238912
• 关键词: Affect; Animals; Area; Aromatic Polycyclic Hydrocarbons; Behavior; Biological Availability; Biological Markers; Body Burden; Chemicals; Communities; Computer Simulation; Consumption; Crabs; Data; Disease Marker; Event; Explosion; Exposure to; Fishes; Food Chain; Food Webs; Future; Goals; Health; Health Alliance; Human; Hydrocarbons; Individual; Life; Longevity; Longitudinal Studies; Louisiana; Malignant Neoplasms; Marines; Mass Fragmentography; Measures; Metabolic; Metabolism; Mexico; Modeling; Natural History; Oils; Organism; Oysters; Pattern; Petroleum; Population; Populations at Risk; Research; Risk; Sampling; Scientist; Seafood; Serum; Shrimp; Source; Stress; Texas; Toxic effect; Universities; Water Pollution; base; bioaccumulation; community based participatory research; data modeling; experience; exposed human population; gulf coast; information model; life history; marine organism; member; metabolomics; research study; response; stressor

The DWH oil spill is perceived to be a risk to human health in coastal communities. This risk will be examined in our project by measuring the concentration of petrogenic polycyclic aromatic hydrocarbons (PAHs) in human serum and its source, the contaminated seafood that they consume. Complementary modeling experiments planned in this project will provide the predictive exposure risks from consuming tainted seafood. Therefore, we will determine the body burden signature of petrogenic PAHs in various marine species with different life histories and metabolic capacities. The signature of PAHs identified in marine animals will be measured in individuals in the fishing communities consuming oil-tainted seafood. The data thus obtained will be utilized in computational modeling of migratory behavior and food web transfers to predict the risk of persistent human exposure. To establish levels of PAHs in marine animals and their impact on human health from long-term exposure to food chain contaminants, we propose the following three specific aims: Specific Aim 1. Biomarkers of Exposure in Marine Animals: To determine the burden of DWH petrogenic PAHs in fishes, shrimps, crabs and oysters collected from various BP Gulf oil spill affected areas using gas chromatography-mass spectrometry (GC-MS)-based approach to provide hydrocarbon signature for DWH oil exposure. Specific Aim 2. Biomarker of Exposure in Humans: To assess human exposure to DWH oil, the most prevalent hydrocarbons identified in marine life (hydrocarbon signature) under Aim1 will be analyzed in human serum samples collected from individuals in our partner communities using GC-MS. Specific Aim 3. Inverse Exposure Modeling: Data obtained from Aims 1 and 2 will be utilized in inverse models of marine food webs of target (exposed) species to determine the risk of exposure of DWH oil to affected communities. The models will combine known information of the natural history of the organisms, the evolving condition and distribution of the contaminants and the co-distribution of the two over the life span of the organisms and their prey. This study will be conducted by an experienced team of scientists from UTMB and Texas A&M University at Galveston to provide collective information about the oil exposure of at-risk populations in a true interdisciplinary fashion. This research will be based on current human exposure data as well as future exposure risks. Our project is developed using substantial community input and will provide the necessary data to confirm exposure to DWH PAHs for various stressor analysis in Project 1 and contribute vitally to Projects 3 (PAH extracts) and 4 (exposure data) to obtain the correlations between exposure and biomarkers of stress and toxicity including early and intermediate markers of diseases (ie. cancer).

DWH漏油事件被认为对沿海社区的人类健康构成了威胁。在我们的项目中，将通过测量人血清及其来源--他们消费的受污染的海鲜--中的成岩多环芳烃(PAHs)浓度来检查这种风险。本项目中计划的补充模拟实验将提供食用受污染海鲜的预测性暴露风险。因此，我们将确定不同生活史和代谢能力的不同海洋物种中成岩多环芳烃的身体负担特征。在海洋动物中发现的多环芳烃的特征将在食用受石油污染的海鲜的渔业社区的个人中进行测量。这样获得的数据将被用于迁徙行为和食物网转移的计算模型，以预测人类持续接触的风险。为了确定长期暴露在食物链污染物中的海洋动物中的多环芳烃水平及其对人类健康的影响，我们提出了以下三个具体目标：具体目标1.海洋动物暴露的生物标志物：利用基于气相色谱-质谱仪(GC-MS)的方法，测定从英国石油公司墨西哥湾石油泄漏影响区域采集的鱼、虾、蟹和牡蛎中DWH成岩多环芳烃的负担，以提供DWH石油暴露的碳氢化合物特征。具体目标2.人类暴露的生物标志物：为了评估人类暴露在DWH石油中，将使用GC-MS分析从我们伙伴社区的个人收集的人类血清样本中，根据Aim1在海洋生物中识别的最常见的碳氢化合物(碳氢化合物特征)。具体目标3.反向暴露模型：从目标1和目标2获得的数据将用于目标(暴露)物种的海洋食物网的反向模型，以确定DWH油暴露到受影响社区的风险。这些模型将结合生物体的自然历史、污染物的演变条件和分布以及两者在生物体及其猎物的生命周期中的共同分布的已知信息。 这项研究将由来自UTMB和加尔维斯顿德克萨斯农工大学的经验丰富的科学家团队进行，以真正的跨学科方式提供有关高危人群石油暴露的集体信息。这项研究将基于当前的人体暴露数据以及未来的暴露风险。我们的项目是利用大量的社区投入开发的，并将提供必要的数据，以确认项目1中各种应激源分析的DWH PAHs暴露，并对 项目3(多环芳烃提取物)和项目4(暴露数据)，以获得暴露与应激和毒性的生物标志物之间的相关性，包括疾病的早期和中期标志物(即。癌症)。