Project 2: Exposure Assessment of Macondo Oil Spill in Affected Communities

项目 2：受影响社区马孔多漏油事件暴露评估

• 批准号: 8469043
• 负责人: G.A. Shakeel Ansari
• 金额: $ 23.89万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8469043
• 关键词: Affect; Animals; Area; Aromatic Polycyclic Hydrocarbons; Behavior; Biological Availability; Biological Markers; Body Burden; Chemicals; Communities; Computer Simulation; Consumption; Crabs; Data; Disease Marker; Event; Explosion; Exposure to; Fishes; Food Chain; Food Webs; Future; Goals; Health; Health Alliance; Human; Hydrocarbons; Individual; Life; Longevity; Longitudinal Studies; Louisiana; Malignant Neoplasms; Marines; Mass Fragmentography; Measures; Metabolic; Metabolism; Mexico; Modeling; Natural History; Oils; Organism; Oysters; Pattern; Petroleum; Population; Populations at Risk; Research; Risk; Sampling; Scientist; Seafood; Serum; Shrimp; Source; Stress; Texas; Toxic effect; Universities; Water Pollution; base; bioaccumulation; community based participatory research; data modeling; experience; exposed human population; gulf coast; information model; life history; marine organism; member; metabolomics; research study; response; stressor

The DWH oil spill is perceived to be a risk to human health in coastal communities. This risk will be examined in our project by measuring the concentration of petrogenic polycyclic aromatic hydrocarbons (PAHs) in human serum and its source, the contaminated seafood that they consume. Complementary modeling experiments planned in this project will provide the predictive exposure risks from consuming tainted seafood. Therefore, we will determine the body burden signature of petrogenic PAHs in various marine species with different life histories and metabolic capacities. The signature of PAHs identified in marine animals will be measured in individuals in the fishing communities consuming oil-tainted seafood. The data thus obtained will be utilized in computational modeling of migratory behavior and food web transfers to predict the risk of persistent human exposure. To establish levels of PAHs in marine animals and their impact on human health from long-term exposure to food chain contaminants, we propose the following three specific aims: Specific Aim 1. Biomarkers of Exposure in Marine Animals: To determine the burden of DWH petrogenic PAHs in fishes, shrimps, crabs and oysters collected from various BP Gulf oil spill affected areas using gas chromatography-mass spectrometry (GC-MS)-based approach to provide hydrocarbon signature for DWH oil exposure. Specific Aim 2. Biomarker of Exposure in Humans: To assess human exposure to DWH oil, the most prevalent hydrocarbons identified in marine life (hydrocarbon signature) under Aim1 will be analyzed in human serum samples collected from individuals in our partner communities using GC-MS. Specific Aim 3. Inverse Exposure Modeling: Data obtained from Aims 1 and 2 will be utilized in inverse models of marine food webs of target (exposed) species to determine the risk of exposure of DWH oil to affected communities. The models will combine known information of the natural history of the organisms, the evolving condition and distribution of the contaminants and the co-distribution of the two over the life span of the organisms and their prey. This study will be conducted by an experienced team of scientists from UTMB and Texas A&M University at Galveston to provide collective information about the oil exposure of at-risk populations in a true interdisciplinary fashion. This research will be based on current human exposure data as well as future exposure risks. Our project is developed using substantial community input and will provide the necessary data to confirm exposure to DWH PAHs for various stressor analysis in Project 1 and contribute vitally to Projects 3 (PAH extracts) and 4 (exposure data) to obtain the correlations between exposure and biomarkers of stress and toxicity including early and intermediate markers of diseases (ie. cancer).

DWH石油泄漏被认为对沿海社区的人类健康构成威胁。在我们的项目中，将通过测量人体血清中的石油多环芳烃（PAHs）浓度及其来源，即他们食用的受污染海鲜来研究这种风险。该项目计划的补充建模实验将提供食用受污染海鲜的预测暴露风险。因此，我们将确定在不同的生活史和代谢能力的各种海洋物种的石油多环芳烃的身体负担签名。在海洋动物中发现的多环芳烃的特征将在食用石油污染海鲜的渔业社区的个体中进行测量。 由此获得的数据将用于迁移行为和食物网转移的计算建模，以预测人类持续接触的风险。为了确定海洋动物体内多环芳烃的水平及其长期暴露于食物链污染物对人类健康的影响，我们提出了以下三个具体目标：具体目标1。海洋动物暴露的生物标志物：使用基于气相色谱-质谱（GC-MS）的方法确定从英国石油公司海湾漏油影响区域收集的鱼类、虾、蟹和牡蛎中DWH岩石成因多环芳烃的负荷，以提供DWH石油暴露的碳氢化合物特征。具体目标2。人类暴露的生物标志物：为了评估人类对DWH油的暴露，将使用GC-MS分析从我们的合作伙伴社区的个人收集的人类血清样本中Aim 1下海洋生物中确定的最常见的碳氢化合物（碳氢化合物特征）。逆曝光建模：从目标1和目标2获得的数据将用于目标（接触）物种的海洋食物网逆模型，以确定接触DWH石油对受影响社区的风险。这些模型将联合收割机生物体自然历史的已知信息、污染物的演变条件和分布以及两者在生物体及其猎物的寿命期间的共同分布。 这项研究将由来自UTMB和德克萨斯A&M大学加尔维斯顿的经验丰富的科学家团队进行，以真正的跨学科方式提供有关风险人群石油暴露的集体信息。这项研究将基于目前的人类接触数据以及未来的接触风险。我们的项目是利用大量的社区投入开发的，并将提供必要的数据，以确认暴露于DWH多环芳烃的项目1中的各种压力源分析，并有助于至关重要的 项目3（多环芳烃提取物）和项目4（暴露数据）旨在获得暴露与应激和毒性生物标志物（包括疾病的早期和中期标志物）之间的相关性。癌症）。