Development of the Basal Telencephalic Limbic System

基底端脑边缘系统的发育

• 批准号: 8040686
• 负责人: JOSHUA G CORBIN
• 金额: $ 36.77万
• 依托单位: CHILDREN'S RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2016-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8040686
• 关键词: Addictive Behavior; Amygdaloid structure; Axon; Behavior Disorders; Behavioral; Biological Assay; Brain; Brain Diseases; Cell Nucleus; Cells; Characteristics; Complex; Development; Disease; Electrophysiology (science); Embryo; Emotional; Functional disorder; Gene Expression; Genes; Genetic; Goals; Human; In Situ Hybridization; Information Storage; Knowledge; Label; Limbic System; Link; Maps; Medial; Molecular; Mus; Neurons; Nuclear Structure; Pattern; Population; Preoptic Areas; Process; Property; Regulation; Reporter; Role; Series; Sex Behavior; Stimulus; Structure; Synapses; Telencephalon; Testing; Tracer; Work; autism spectrum disorder; base; combinatorial; developmental disease; gene function; insight; loss of function; molecular marker; neuronal patterning; patch clamp; postnatal; progenitor; research study; response; transcription factor

DESCRIPTION (provided by applicant): This renewal application is focused on the study of the development of the basolateral complex and medial nucleus of the amygdala. Collectively these nuclei regulate major aspects of limbic system function. Our previous studies have identified distinct progenitor pools in the developing telencephalon that contribute to postnatal neuronal cell diversity in these amygdala subdivisions. Based on this work, in this project we will test two hypotheses. First, we will test the hypothesis that embryonic transcriptional factor expression diversity within amygdala progenitor pools underlies differential postnatal amygdala neuronal subtype fate and patterns of axonal connectivity. Second, we will test the hypothesis that key transcription factors that are expressed in these progenitor domains are required for the development and/or connectivity of postnatal amygdala neurons that are derived from these populations. Testing of these hypotheses will be accomplished using a combination cutting edge approaches including genetic fate mapping, electrophysiology, axonal tracing and conditional loss of function. PUBLIC HEALTH RELEVANCE: The mammalian amygdala is a central structure of the brain's limbic system, a brain circuit that coordinates appropriate behavioral responses to stimuli with emotional and motivational salience. Amygdala dysfunction is associated with numerous brain disorders including addictive behavior and developmental disorders such as autism spectrum disorders. This proposal is directed toward understanding the genetic and cellular basis of amygdala development, and thus will provide valuable insight into human disorders in which amygdala function is altered.

描述（由申请人提供）：本次更新申请的重点是研究杏仁核基底外侧复合体和内侧核的发育。这些核团共同调节边缘系统功能的主要方面。我们以前的研究已经确定了不同的祖细胞池在发展中的端脑，有助于出生后的神经元细胞多样性，在这些杏仁核亚部。在此基础上，我们将在本项目中验证两个假设。首先，我们将测试的假设，胚胎转录因子表达的多样性杏仁核祖池的基础差异出生后杏仁核神经元亚型的命运和轴突连接模式。第二，我们将测试的假设，在这些祖域中表达的关键转录因子所需的发育和/或出生后的杏仁核神经元，来自这些人口的连接。这些假设的测试将使用组合尖端方法完成，包括遗传命运映射，电生理学，轴突追踪和条件性功能丧失。 公共卫生关系：哺乳动物杏仁核是大脑边缘系统的中心结构，边缘系统是一种大脑回路，可以协调对具有情感和动机显着性的刺激的适当行为反应。杏仁核功能障碍与许多脑部疾病有关，包括成瘾行为和发育障碍，如自闭症谱系障碍。这一建议是针对了解杏仁核发育的遗传和细胞基础，从而将提供有价值的见解杏仁核功能改变的人类疾病。