Development of the Basal Telencephalic Limbic System

基底端脑边缘系统的发育

• 批准号: 8040686
• 负责人: JOSHUA G CORBIN
• 金额: $ 36.77万
• 依托单位: CHILDREN'S RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2016-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8040686
• 关键词: Addictive Behavior; Amygdaloid structure; Axon; Behavior Disorders; Behavioral; Biological Assay; Brain; Brain Diseases; Cell Nucleus; Cells; Characteristics; Complex; Development; Disease; Electrophysiology (science); Embryo; Emotional; Functional disorder; Gene Expression; Genes; Genetic; Goals; Human; In Situ Hybridization; Information Storage; Knowledge; Label; Limbic System; Link; Maps; Medial; Molecular; Mus; Neurons; Nuclear Structure; Pattern; Population; Preoptic Areas; Process; Property; Regulation; Reporter; Role; Series; Sex Behavior; Stimulus; Structure; Synapses; Telencephalon; Testing; Tracer; Work; autism spectrum disorder; base; combinatorial; developmental disease; gene function; insight; loss of function; molecular marker; neuronal patterning; patch clamp; postnatal; progenitor; research study; response; transcription factor

DESCRIPTION (provided by applicant): This renewal application is focused on the study of the development of the basolateral complex and medial nucleus of the amygdala. Collectively these nuclei regulate major aspects of limbic system function. Our previous studies have identified distinct progenitor pools in the developing telencephalon that contribute to postnatal neuronal cell diversity in these amygdala subdivisions. Based on this work, in this project we will test two hypotheses. First, we will test the hypothesis that embryonic transcriptional factor expression diversity within amygdala progenitor pools underlies differential postnatal amygdala neuronal subtype fate and patterns of axonal connectivity. Second, we will test the hypothesis that key transcription factors that are expressed in these progenitor domains are required for the development and/or connectivity of postnatal amygdala neurons that are derived from these populations. Testing of these hypotheses will be accomplished using a combination cutting edge approaches including genetic fate mapping, electrophysiology, axonal tracing and conditional loss of function. PUBLIC HEALTH RELEVANCE: The mammalian amygdala is a central structure of the brain's limbic system, a brain circuit that coordinates appropriate behavioral responses to stimuli with emotional and motivational salience. Amygdala dysfunction is associated with numerous brain disorders including addictive behavior and developmental disorders such as autism spectrum disorders. This proposal is directed toward understanding the genetic and cellular basis of amygdala development, and thus will provide valuable insight into human disorders in which amygdala function is altered.

描述（由申请人提供）：此更新申请的重点是研究杏仁核基底外侧复合体和内侧核的发育。这些核共同调节边缘系统功能的主要方面。我们之前的研究已经在发育中的端脑中发现了不同的祖细胞池，这些祖细胞有助于这些杏仁核分支的出生后神经元细胞多样性。基于这项工作，在这个项目中，我们将检验两个假设。首先，我们将验证杏仁核祖细胞池中胚胎转录因子表达多样性是出生后杏仁核神经元亚型命运和轴突连接模式差异的基础。其次，我们将验证在这些祖结构域中表达的关键转录因子是来自这些群体的出生后杏仁核神经元发育和/或连接所必需的假设。这些假设的测试将使用包括遗传命运图谱、电生理学、轴突追踪和条件功能丧失在内的前沿方法来完成。