High Throughput Functional Genomics and Proteomics

高通量功能基因组学和蛋白质组学

• 批准号: 8062906
• 负责人: JOSEPH L. DERISI
• 金额: $ 149.42万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-13 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8062906
• 关键词: Affinity; Amino Acid Sequence; Bioinformatics; Biological Assay; Collaborations; Computer Analysis; Computer software; Data; Databases; Development; Enterovirus; Enterovirus Infections; Evolution; Generations; Genetic; Genomics; Goals; Grant; Health Services Research; Homeostasis; Human Resources; Individual; Infection; Informatics; Institutes; Instruction; Libraries; Maps; Mass Spectrum Analysis; Measurement; Methodology; Modeling; Modification; Mutation; Participant; Pathogenesis; Peptide Sequence Determination; Phosphorylation; Population; Positioning Attribute; Post-Translational Protein Processing; Process; Proteins; Proteomics; Public Health; RNA; RNA Interference; Research; Research Infrastructure; Research Support; Ribosomes; Role; Screening procedure; Sequence Analysis; Services; Structural Models; Structure; Technology; Ubiquitination; Ursidae Family; Viral; Viral Genome; Work; base; comparative; design; fitness; functional genomics; genome-wide; high throughput screening; programs; protein complex; research study; tool

PROJECT SUMMARY (See instructions): The High-Throughput Functional Genomics, Proteomics, and Computational Core (referred to as "Core B") of this program project proposal has four distinct research support goals that are designed to enable the Individual objectives as described In each project. These research roles are as follows. First, Core B will provide high-throughput mass spectrometry services for the purpose of establishing viral and host proteinprotein Interaction maps. Second, Core B will provide advanced computational research support, by bringing modern bioinformatic approaches to protein sequence, structure, and function analysis to bear on the expected deluge of functional data this proposal will generate. Third, Core B will provide leading-edge ultra deep sequencing support to the program project. A major focus of the proposal is the analysis of viral diversity, population structure and plasticity, especially with respect to the evolution of quasi-specles. Ultra deep sequencing (such as that provided by lllumina Solexa technology) allows quantitative and precise measurements of genome-wide genetic dynamics at a depth and accuracy that was unthinkable just a few years ago. The field of deep sequencing, which Includes the methodologies for library generation, the actual sequencing, and the downstream sequence analysis, are rapidly changing. This section of the core will actively research and optimize approaches and Informatics associated with sequencing to support the goals of the program project. Fourth, Core B will provide high-throughput RNAi screening support to the program. In conjunction with the Chandra and Young labs, personnel of Core B will work closely with the Chandra and Young labs for the generation of RNAi libraries and the actual screening.

项目总结（见说明）： 本计划项目提案的高通量功能基因组学、蛋白质组学和计算核心（简称“核心B”）有四个不同的研究支持目标，旨在实现每个项目中描述的个人目标。这些研究角色如下。首先，Core B将提供高通量质谱服务，以建立病毒和宿主蛋白质蛋白质 互动地图。第二，核心B将提供先进的计算研究支持，通过将现代生物信息学方法引入蛋白质序列、结构和功能分析，以应对该提案将产生的预期的大量功能数据。三是Core B将为该计划项目提供前沿的超深度测序支持。该提案的一个主要重点是分析病毒的多样性，种群结构和可塑性，特别是关于准物种的进化。超 深度测序（例如由Illumina Solexa技术提供的测序）允许以仅在几年前不可想象的深度和准确度定量和精确地测量全基因组遗传动态。深度测序领域，包括文库生成方法、实际测序和下游序列分析，正在迅速变化。核心部分的这一部分将积极研究和优化与测序相关的方法和信息学，以支持目标 的项目。第四，Core B将为该项目提供高通量RNAi筛选支持。 Core B的工作人员将与Chandra和Young实验室密切合作，以生成RNAi文库并进行实际筛选。