High Throughput Functional Genomics and Proteomics

项目总结(见说明)： 本计划项目建议书的高通量功能基因组学、蛋白质组学和计算核心(简称“核心B”)有四个不同的研究支持目标，旨在实现每个项目中描述的个别目标。这些研究角色如下。首先，核心B将提供高通量质谱学服务，目的是建立病毒和宿主蛋白 交互地图。其次，核心B将提供先进的计算研究支持，将现代生物信息学方法应用于蛋白质序列、结构和功能分析，以应对这项提议将产生的预期功能数据洪流。第三，酷睿B将为计划项目提供前沿的超深测序支持。该提案的一个主要重点是分析病毒多样性、种群结构和可塑性，特别是关于类斑点的进化。超 深度测序(如llLumina Solexa技术提供的测序)能够以几年前不可想象的深度和准确性对全基因组遗传动态进行定量和精确的测量。深度测序领域正在迅速变化，包括文库生成、实际测序和下游序列分析的方法。核心的这一部分将积极研究和优化与排序相关的方法和信息学，以支持目标 该计划项目的。第四，Core B将为该计划提供高通量RNAi筛选支持。 与Chandra和Young实验室合作，Core B的人员将与Chandra和Young实验室密切合作，生成RNAi文库并进行实际筛选。

PROJECT SUMMARY (See instructions): The High-Throughput Functional Genomics, Proteomics, and Computational Core (referred to as "Core B") of this program project proposal has four distinct research support goals that are designed to enable the Individual objectives as described In each project. These research roles are as follows. First, Core B will provide high-throughput mass spectrometry services for the purpose of establishing viral and host proteinprotein Interaction maps. Second, Core B will provide advanced computational research support, by bringing modern bioinformatic approaches to protein sequence, structure, and function analysis to bear on the expected deluge of functional data this proposal will generate. Third, Core B will provide leading-edge ultra deep sequencing support to the program project. A major focus of the proposal is the analysis of viral diversity, population structure and plasticity, especially with respect to the evolution of quasi-specles. Ultra deep sequencing (such as that provided by lllumina Solexa technology) allows quantitative and precise measurements of genome-wide genetic dynamics at a depth and accuracy that was unthinkable just a few years ago. The field of deep sequencing, which Includes the methodologies for library generation, the actual sequencing, and the downstream sequence analysis, are rapidly changing. This section of the core will actively research and optimize approaches and Informatics associated with sequencing to support the goals of the program project. Fourth, Core B will provide high-throughput RNAi screening support to the program. In conjunction with the Chandra and Young labs, personnel of Core B will work closely with the Chandra and Young labs for the generation of RNAi libraries and the actual screening.