Synaptic Transmission: Modulation, Plasticity And Effects Of Drugs Of Abuse

突触传递：调节、可塑性和滥用药物的影响

• 批准号: 8344686
• 负责人: David M Lovinger
• 金额: $ 156.58万
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8344686
• 关键词: Acute; Adult; Affect; Alcohol abuse; Alcohol consumption; Alcohol dependence; Alcoholic Intoxication; Alcohols; Animal Model; Animals; Axon; Behavior; Behavior Control; Behavioral; Blood alcohol level measurement; Brain; Brain region; CNR1 gene; Calcium Channel; Cell Nucleus; Chronic; Cognition; Corpus striatum structure; Decision Making; Dendrites; Dendritic Spines; Dependence; Development; Dorsal; Drug Exposure; Elements; Endocannabinoids; Ethanol; Excitatory Synapse; Exposure to; Fetal Alcohol Spectrum Disorder; Frequencies; G-Protein-Coupled Receptors; Glutamates; Goals; Habits; Human; Individual; Intake; Interneurons; Intoxication; Investigation; Knowledge; L-Type Calcium Channels; Laboratories; Learning; Life; Light; Lobular Neoplasia; Long-Term Depression; Macaca; Mediating; Membrane Potentials; Membrane Proteins; Monkeys; Mus; Neurons; Neurosciences; Output; Pattern; Pharmaceutical Preparations; Pharmacology; Physiological; Play; Pregnancy; Process; Production; Prosencephalon; Receptor Activation; Reporting; Research; Research Personnel; Rhodopsin; Risk Factors; Role; Shapes; Signal Transduction; Site; Sorting - Cell Movement; Synapses; Synaptic Transmission; Synaptic plasticity; Techniques; Work; addiction; alcohol behavior; alcohol effect; alcohol seeking behavior; alcohol use disorder; base; caudate nucleus; chronic alcohol ingestion; drinking; drug of abuse; early onset; fetal; habit learning; in vivo; interest; neural circuit; neuroadaptation; neuronal cell body; neuronal excitability; neuroregulation; postnatal; postsynaptic; presynaptic; putamen; relating to nervous system; research study; response; synaptic depression; transmission process; vapor; voltage

Research within the Laboratory for Integrative Neuroscience, Section on Synaptic Pharmacology, continues to focus on mechanisms underlying neuromodulation and plasticity and the effects of alcohol and other drugs of abuse on these neuronal functions. Our main interest is the function of the dorsal striatum (DS), a brain region involved in action control and selection, as well as action learning. Long-term depression at striatal GABAergic synapses We have continued our studies of long-term synaptic depression (LTD) at synapses in striatum. Previous work in the laboratory indicated that LTD occurs at GABAergic synapses intrinsic to the striatum. This LTD can be induced by afferent activation at low-moderate frequencies, and requires endocannabinoid signaling. The two predominant GABAergic inputs to striatal projection neurons (medium spiny neurons or MSNs) come from axon collaterals of other MSNs that synapse mainly on the MSN dendrites, and from the fast-spiking interneurons that synapse mainly near the MSN soma. We have used optogenetic techniques to activate the two GABAergic inputs independently, by expressing channel rhodopsin 2 selectively in the two neuronal subtypes and activating this channel with light. This approach has revealed two types of LTD at these GABAergic synapses. When the membrane potential of the postsynaptic MSN is near the normal resting potential called the "down-state", LTD occurs at both sets of inputs, and does not require activation of voltage-gated calcium channels. However, when the MSN membrane potential is depolarized to values near the "up-states" seen in vivo, LTD occurs exclusively at MSN-MSN synapses, and appears to involve L-type calcium channel activation. Under all conditions, LTD is mediated by endocannabinoids acting at CB1 receptors. Current experiments are exploring the different mechanisms involved in up-state versus down-state GABAergic LTD. These studies have revealed that endocannabinoids can produce differential patterns of synaptic plasticity depending on the afferent input and the state of the postsynaptic neuron. These mechanisms have the potential to shape striatal output in multiple ways by differentially affecting inhibition near excitatory synapses on the MSN dendrites, or neuronal activation controlled by GABAergic synapses near the soma. Ethanol actions at striatal GABAergic synapses We are also continuing our studies of ethanol (EtOH) effects on GABAergic synaptic transmission in dorosolateral (DLS) and dorsomedial striatum (DMS). Our observation that EtOH inhibits GABAergic synaptic transmission in DLS MSNs via a presynaptic mechanism, while potentiating transmission in DMS, suggests differential mechanisms of EtOH action in the two striatal subregions. We are currently using pharmacological approaches, as well as the optogenetic techniques described above, to determine if these differential EtOH effects occur at different afferent inputs to MSNs. These opposing effects are surprising, and may indicate that EtOH suppresses the output of the DMS that is important for goal-directed actions, while enhancing the output of DLS which is involved in habit formation. Our continuing studies of chronic EtOH effects in mice and macaque monkeys have revealed synaptic changes that could contribute to prolonged, habitual alcohol use and abuse. In both model organisms, chronic EtOH exposure leads to a maintained decrease in GABAergic synaptic transmission in DLS and DMS (roughly equivalent to putamen and caudate nucleus, respectively, in monkeys). The findings in monkey are particularly interesting, as the alcohol drinking in this model organism is heavy and prolonged, sharing many features with human drinking. One notable feature of drinking in these monkeys is that animals develop highly regular alcohol intake with increased duration of intake bouts after 2 years of drinking, and the blood alcohol levels they achieve gradually escalate over a three year period. The decreased GABAergic transmission we have observed in putamen MSNs from these monkeys is strongly correlated with the average alcohol intake for each individual monkey. We have also observed increases in glutamatergic synaptic transmission and increased numbers of dendritic spines (the sites of glutamatergic synapses) in the putamen of the chronic EtOH-drinking monkeys. Intrinsic neuronal excitability is also increased after prolong drinking in the putamen MSNs. Recent findings have extended our knowledge by showing that GABAergic synaptic changes are greater in monkeys with earlier onset of drinking, a known risk factor for human alcohol use disorders. The synaptic changes that we have observed in these monkeys appear to render the putamen nucleus hyperexcitable, due to a combination of decreased GABAergic inhibition and increased intrinsic and glutamatergic synaptic excitability. Given the role of the putamen and associated circuitry in development and production of repetitive habitual actions, we hypothesize that the hyperexcitable putamen contributes to maintained, invariant patterns of drinking with high alcohol loading observed in the monkeys. We are also continuing to study effects of fetal/early-postnatal EtOH exposure on striatal function. This project was stimulated by reports implicating altered corticostriatal function in humans with fetal alcohol spectrum disorder (FASD). We are using vapor exposure to EtOH during gestation and the early postnatal period. Electrophysiological experiments have revealed decreased GABAergic synaptic transmission in DLS MSNs examined in adult mice following fetal/early postnatal EtOH exposure. The normal decrease in GABAergic transmission produced by acute exposure to EtOH is lost in these mice, suggesting development of some sort of tolerance to EtOH actions. Interestingly, the synaptic depression normally produced by CB1 receptor activation is also lost in the fetal/early postnatal EtOH-exposed mouse DLS MSNs. Preliminary studies indicate that this effect is selective for CB1 in comparison to to other presynaptic G protein-coupled receptors, and that an abnormal tonic activation of CB1 receptors by eCBs may occur as a consequence of this early life EtOH exposure. Our parallel behavioral studies indicate that this early-life EtOH exposure also impairs habit learning in the mice, and disrupts the associated changes in striatal neuronal activity. These experiments have revealed changes in striatal synaptic transmission that could contribute to abnormalities in the corticostriatal circuitry seen in FASD.

综合神经科学实验室突触药理学部分的研究继续关注神经调节和可塑性的潜在机制，以及酒精和其他滥用药物对这些神经元功能的影响。我们的主要兴趣是背纹状体（DS）的功能，这是一个涉及动作控制和选择以及动作学习的大脑区域。